Promoter-specific regulation of MyoD binding and signal transduction cooperate to pattern gene expression
Promoter-specific regulation of MyoD binding and signal transduction cooperate to pattern gene expression
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DOI：
10.1016/s1097-2765(02)00481-1
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发表时间：
2002-03-01
期刊：
影响因子：
16
通讯作者：
Tapscott, SJ
中科院分区：
文献类型：
--
作者：
Bergstrom, DA;Penn, BH;Tapscott, SJ
我们使用表达阵列和染色质免疫沉淀试验来证明肌生成由MyoD调控的基因表达的离散子程序组成。大约5%的基因在特定的时间序列中改变表达，超过1%的基因受MyoD的调控，而不合成额外的转录因子。MyoD调节在肌发生过程中不同时间表达的基因，并且MyoD结合的启动子特异性调节是基因表达模式化的主要机制。此外，p38激酶活性对于受MyoD调节的基因的有限子集的表达是必需的，但对于MyoD结合不是必需的。鉴定不同的分子机制，调节肌生成的离散子程序，应有助于正常发育和疾病的分化分析。
We used expression arrays and chromatin immunoprecipitation assays to demonstrate that myogenesis consists of discrete subprograms of gene expression regulated by MyoD. Approximately 5% of assayed genes alter expression in a specific temporal sequence, and more than 1% are regulated by MyoD without the synthesis of additional transcription factors. MyoD regulates genes expressed at different times during myogenesis, and promoter-specific regulation of MyoD binding is a major mechanism of patterning gene expression. In addition, p38 kinase activity is necessary for the expression of a restricted subset of genes regulated by MyoD, but not for MyoD binding. The identification of distinct molecular mechanisms that regulate discrete subprograms of myogenesis should facilitate analyses of differentiation in normal development and disease.