MUTANT PHENOTYPES SUPPORT A TRANS-SPLICING MECHANISM FOR THE EXPRESSION OF THE TRIPARTITE PSAA GENE IN THE C-REINHARDTII CHLOROPLAST
MUTANT PHENOTYPES SUPPORT A TRANS-SPLICING MECHANISM FOR THE EXPRESSION OF THE TRIPARTITE PSAA GENE IN THE C-REINHARDTII CHLOROPLAST
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DOI：
10.1016/0092-8674(88)90432-1
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发表时间：
1988-03-25
期刊：
影响因子：
64.5
通讯作者：
ROCHAIX, JD
中科院分区：
文献类型：
--
作者：
CHOQUET, Y;GOLDSCHMIDTCLERMONT, M;ROCHAIX, JD
绿色单细胞衣藻叶绿体psaA基因由三个外显子组成，它们是从不同的链转录的。许多核和叶绿体突变体，缺乏光系统I活性的分析表明，rouggly四分之一的人是专门影响psaA mRNA成熟。这些突变体可以分为三个表型类，基于它们不能进行一个或两个剪接反应。这些数据表明，这三个外显子作为前体独立转录，这些前体通常以反式组装，并且剪接反应可以以任何顺序发生。虽然一些叶绿体突变可以顺式发挥作用，但落入几个互补组的核突变可能会影响组装psaA mRNA所需的特定因子。
The chloroplast psaA gene of the green unicellular alga Chlamydomonas reinhardtii consists of three exons that are transcribed from different strands. Analysis of numerous nuclear and chloroplast mutants that are deficient in photosystem I activity reveals that rougly one-quarter of them are specifically affected in psaA mRNA maturation. These mutants can be grouped into three phenotypic classes, based on their inability to perform either one or both splicing reactions. The data indicate that the three exons are transcribed independently as precursors which are normally assembled in trans and that the splicing reactions can occur in either order. While some chloroplast mutations could act in cis, the nuclear mutations that fall into several complementation groups probably affect factors specifically required for assembling psaA mRNA.