Surface-structured bacterial cellulose loaded with hUSCs accelerate skin wound healing by promoting angiogenesis in rats
Surface-structured bacterial cellulose loaded with hUSCs accelerate skin wound healing by promoting angiogenesis in rats
复制标题
负载 hUSC 的表面结构细菌纤维素通过促进大鼠血管生成加速皮肤伤口愈合
DOI：
10.1016/j.bbrc.2019.06.161
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发表时间：
2019-09-03
影响因子：
3.1
通讯作者：
Wu, Qing-kai
中科院分区：
文献类型：
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作者：
Cao, Yi-meng;Liu, Meng-yu;Wu, Qing-kai
促进伤口愈合是临床医学研究的重要领域之一。本研究旨在评估一种新的表面结构细菌纤维素（S-BC）生物材料与人尿源性干细胞（hUSCs）在伤口愈合中的潜在应用。在体外，将EA.hy926接种于结构化/非结构化细菌纤维素上，观察EA.hy926在有/无条件培养基的细菌纤维素上的生长情况，探讨细菌纤维素表面结构和hUSCs- cm对血管内皮细胞生长的影响。在体内，我们分别在BC组、S-BC组、hUSCs组、BC + hUSCs组、S-BC + hUSCs组对大鼠全层皮肤缺损模型创面愈合的影响进行了评价。体外实验表明，S-BC表面结构能促进EA.hy926的生长和存活，且hscs - cm能进一步促进EA.hy926在S-BC上的增殖。体内实验结果显示，与对照组相比，BC组、S-BC组、hUSCs组的创面愈合速度明显加快，并伴有更快的再上皮化、胶原生成和新生血管形成。值得注意的是，S-BC对创面愈合的效果优于BC， S-BC + hUSCs对创面愈合的效果优于BC + hUSCs。此外，S-BC联合hUSCs对伤口的影响优于S-BC或hUSCs单独治疗。上述结果提示，S-BC与hUSCs联合使用可通过促进血管生成促进皮肤创面愈合。干细胞和生物材料表面结构的结合可能为解决临床伤口愈合问题提供新的途径。(C) 2019 Elsevier Inc.版权所有。
Promotion of wound healing is one of the most important fields in clinical medical research. This study aimed to evaluate the potential use of a new surface-structured bacterial cellulose(S-BC) biomaterial with human urine-derived stem cells (hUSCs) for wound healing. In vitro, EA.hy926 were inoculated on structured/non-structured bacterial cellulose, and the growth of EA.hy926 on bacterial cellulose in medium with/without conditioned medium of the hUSCs were observed to explore the effect of bacterial cellulose's surface structure and hUSCs-CM on vascular endothelial cell growth. In vivo, we covered wound surface with various BC materials and/or injected the hUSCs into the wound site on group BC, group S-BC, group hUSCs, group BC + hUSCs, group S-BC + hUSCs to evaluate the effect of S-BC and hUSCs on wound healing in rat full-thickness skin defect model. In vitro study, surface structure of S-BC could promote the growth and survival of EA.hy926, and the hUSCs-CM could further promote the proliferation of EA.hy926 on S-BC. In vivo study, wound healing rate of the group BC, group S-BC, group hUSCs was significantly accelerated, accompanied by faster re-epithelialization, collagen production and neovascularization than control group. It is note worthy that the effect of S-BC on wound healing was better than BC, the effect of S-BC + hUSCs on wound healing was better than BC + hUSCs. Moreover, the effect of S-BC combined with hUSCs on wound is better than treated with S-BC or hUSCs alone. All the findings suggest that the combination of S-BC and hUSCs could facilitate skin wound healing by promoting angiogenesis. This combination of the role of stem cells and biomaterial surface structures may provide a new way to address clinical wound healing problems. (C) 2019 Elsevier Inc. All rights reserved.