Functional diversity of cytochrome P450s of the white-rot fungus Phanerochaete chrysosporium
Functional diversity of cytochrome P450s of the white-rot fungus Phanerochaete chrysosporium
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DOI：
10.1016/j.bbrc.2004.09.062
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发表时间：
2004-11-05
影响因子：
3.1
通讯作者：
Wariishi, H
中科院分区：
文献类型：
--
作者：
Matsuzaki, F;Wariishi, H
研究了白腐菌黄孢原毛平革菌(Phanerochaete Chrysosporium)细胞色素P450(P450s)的功能多样性。一系列已知为其他生物的P450底物的化合物被用于黄孢原毛霉的代谢研究。首次利用黄孢原毛霉对苯甲酸、樟脑、1，8-桉醇、肉桂酸、对香豆酸、香豆素、异丙苯、1，12-十二醇、1-十二醇、4-乙氧基苯甲酸和7-乙氧基香豆素的代谢转化进行了研究。1-十二醇在7个不同的位置羟化生成1，12-，1，11-，1，10-，1，9-，1，8-，1，7-和1，6-十二醇。还考察了P450抑制剂胡椒基丁醚对1-十二醇真菌转化的影响，结果表明胡椒基丁醚以浓度依赖的方式抑制1-十二醇的羟化反应。在11个底物中，共测定了23个羟基化反应和2个脱乙基反应，其中6个新产物含有羟基的位置。综上所述，真菌P450具有多种独特的功能。(C)2004 Elsevier Inc.保留所有权利。
The functional diversity of cytochrome P450s (P450s) of the white-rot basidiomycete, Phanerochaete chrysosporium, was studied. A series of compounds known to be P450 substrates of other organisms were utilized for metabolic studies of P. chrysosporium. Metabolic conversions of benzoic acid, camphor, 1,8-cineol, cinnamic acid, p-coumaric acid, coumarin, cumene, 1,12-dodecanediol, 1-dodecanol, 4-ethoxybenzoic acid, and 7-ethoxycoumarin were observed with P. chrysosporium for the first time. 1-Dodecanol was hydroxylated at seven different positions to form 1,12-, 1,11-, 1,10-, 1,9-, 1,8-, 1,7-, and 1,6-dodecandiols. The effect of piperonyl butoxide, a P450 inhibitor, on the fungal conversion of 1-dodecanol was also investigated, indicating that hydroxylation reactions of 1-dodecanol were inhibited by piperonyl butoxide in a concentration-dependent manner. With 11 substrates, 23 hydroxylation reactions and 2 deethylation reactions were determined and 6 products were new with the position of hydroxyl group incorporated. In conclusion, fungal P450s were shown to have diverse and unique functions. (C) 2004 Elsevier Inc. All rights reserved.