Histogenesis-specific expression of fibroblast activation protein and dipeptidylpeptidase-IV in human bone and soft tissue tumours.
Histogenesis-specific expression of fibroblast activation protein and dipeptidylpeptidase-IV in human bone and soft tissue tumours.
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DOI：
10.1111/j.1365-2559.2009.03399.x
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发表时间：
2009-10
期刊：
影响因子：
6.4
通讯作者：
Kokubun S
中科院分区：
文献类型：
--
作者：
Dohi O;Ohtani H;Hatori M;Sato E;Hosaka M;Nagura H;Itoi E;Kokubun S
成纤维细胞活化蛋白（FAP）/二肽基肽酶-II（Seprase）和二肽基肽酶 - IV（DPP - IV）/CD26是丝氨酸整合膜蛋白酶。它们参与组织重塑、癌症侵袭和转移，这些机制存在争议。目的是确定在人骨和软组织肿瘤中表达FAP和DPP - IV的细胞类型，并确定FAP和DPP - IV的表达与肿瘤恶性潜能之间是否存在任何相关性。
本研究分析了25例恶性和13例良性人骨和软组织肿瘤的原位表达。逆转录聚合酶链反应分析证实了所有个体中FAP和DPP - IV的mRNA表达。使用预固定冷冻切片的免疫组织化学显示，FAP在低度肌纤维母细胞肉瘤、骨肉瘤的纤维母细胞成分和恶性纤维组织细胞瘤中呈阳性，但在尤文肉瘤和横纹肌肉瘤中呈阴性。DPP - IV显示出相似的免疫组织化学结果。在良性肿瘤中，非骨化性纤维瘤、韧带样瘤和软骨母细胞瘤均表达FAP和DPP - IV。巨细胞在巨细胞瘤中表达DPP - IV。
我们的数据表明，FAP和DPP - IV在与活化的成纤维细胞和/或肌纤维母细胞组织发生相关的骨和软组织肿瘤细胞中持续表达，无论其恶性程度如何。DPP - IV也在单核细胞 - 巨噬细胞谱系细胞中表达。
Fibroblast activation protein (FAP)/seprase and dipeptidylpeptidase-IV (DPP-IV)/CD26 are serine integral membrane proteases. They are involved in tissue remodelling, cancer invasion and metastases, mechanisms that are controversial. The aim was to identify cell types that express FAP and DPP-IV in human bone and soft tissue tumours, and to determine whether there are any correlations between the expression of FAP and DPP-IV and the malignant potential of tumours. This study analysed in situ expression in 25 malignant and 13 benign human bone and soft tissue tumours. Reverse transcriptase-polymerase chain reaction analyses confirmed mRNA expression of FAP and DPP-IV in all individuals. Immunohistochemistry using pre-fixed frozen sections revealed that FAP was positive in low-grade myofibroblastic sarcoma, the fibroblastic component of osteosarcomas, and malignant fibrous histiocytomas, but negative in Ewing’s sarcomas and rhabdomyosarcomas. DPP-IV showed similar immunohistochemical results. Among benign tumours, non-ossifying fibromas, desmoid tumours and chondroblastomas expressed both FAP and DPP-IV. Giant cells expressed DPP-IV in giant cell tumours. Our data suggest that FAP and DPP-IV are consistently expressed in bone and soft tissue tumour cells that are histogenetically related to activated fibroblasts and/or myofibroblasts, irrespective of their malignancy. DPP-IV is also expressed in monocyte–macrophage lineage cells.
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影响因子：
8
作者：
Ramirez-Montagut, T;Blachere, NE;Houghton, AN
通讯作者：
Houghton, AN
DOI：
10.1073/pnas.91.12.5657
发表时间：
1994-06-07
影响因子：
11.1
作者：
SCANLAN, MJ;RAJ, BKM;RETTIG, WJ
通讯作者：
RETTIG, WJ
影响因子：
13.5
作者：
Levy, MT;McCaughan, GW;Gorrell, MD
通讯作者：
Gorrell, MD
影响因子：
6.4
作者：
Montgomery, E;Fisher, C
通讯作者：
Fisher, C
影响因子：
4.8
作者：
Cheng, HC;Abdel-Ghany, M;Pauli, BU
通讯作者：
Pauli, BU