Cytokine-activated endothelium recruits osteoclast precursors.
Cytokine-activated endothelium recruits osteoclast precursors.
复制标题
细胞因子激活的内皮招募破骨细胞前体。
DOI：
10.1210/endo.142.4.8204
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发表时间：
2001
期刊：
影响因子：
4.8
通讯作者：
M. Helfrich
中科院分区：
文献类型：
--
作者：
N. McGowan;E. J. Walker;H. Macpherson;S. Ralston;M. Helfrich
破骨细胞前体通过脉管系统到达破骨细胞形成和重塑的部位，因此在迁移到骨表面之前注定要遇到内皮。在这里，我们研究了内皮细胞可能参与调节破骨细胞前体募集到骨吸收部位的假设。通过在补充RANKLigand、M-CSF、1，25（OH）（2）-维生素D（3）、地塞米松和前列腺素E（2）的21天培养物中形成成熟破骨细胞的能力，鉴定人外周血中的破骨细胞前体。在对照条件下，很少破骨细胞前体粘附于内皮细胞（人骨髓来源的内皮细胞系BMEC-1）。然而，用再吸收刺激细胞因子IL-1 β和TNF α处理的BMEC-1细胞耗尽了所有破骨细胞前体的PBMC群体。这些结果提供了破骨细胞前体可以粘附于内皮的第一个证据，并表明内皮细胞在破骨细胞前体向骨吸收位点的募集中可能发挥重要作用。
Osteoclast precursors reach sites of osteoclast formation and remodelling via the vasculature and are therefore destined to encounter endothelium before migrating to the bone surface. Here we investigated the hypothesis that endothelium may be involved in the regulation of osteoclast precursor recruitment to sites of bone resorption. Osteoclast precursors in human peripheral blood were identified by their ability to form mature osteoclasts in 21-day cultures supplemented with RANKLigand, M-CSF, 1,25(OH)(2)-vitamin D(3), dexamethasone and prostaglandin E(2). Under control conditions few osteoclast precursors adhered to endothelial cells (the human bone marrow-derived endothelial cell line BMEC-1). However, BMEC-1 cells treated with the resorption stimulating cytokines IL-1beta and TNFalpha depleted the PBMC population of all osteoclast precursors. These results provide the first evidence that osteoclast precursors can adhere to endothelium and suggest that endothelium could play an important role in the recruitment of osteoclast precursors to sites of bone resorption.