The Immunological Proteome Resource.
The Immunological Proteome Resource.
复制标题
免疫蛋白质组资源。
DOI：
10.1038/s41590-023-01483-4
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发表时间：
2023
影响因子：
30.5
通讯作者：
Brenes AJ
中科院分区：
文献类型：
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作者：
Brenes AJ
免疫蛋白质组资源（ImmPRes; http：//immpres. co. uk/）。ImmPRes旨在提供免疫细胞蛋白质组的深入高质量图谱。这是一项受ImmGen 1等资源启发的倡议，其中对免疫细胞中RNA表达的系统分析产生了关于淋巴细胞群体的宝贵生物学见解。ImmPRes绘制免疫细胞的蛋白质组而不是转录组，这很重要，因为蛋白质合成和降解速率的变化意味着mRNA水平并不总是细胞蛋白质丰度的有效预测因子。因此，有明确的例子之间的不一致的mRNA和蛋白质丰度的T细胞，反映了转录后机制的能力，以控制T细胞蛋白质组3。由于蛋白质是构成细胞并控制几乎所有代谢过程和调节机制的分子，因此细胞蛋白质组的表征对于理解淋巴细胞身份具有巨大价值。幸运的是，高分辨率质谱技术的进步使得定量表征细胞蛋白质组变得可行。该技术可用于探索免疫细胞在对免疫或环境刺激做出反应时，或在关键信号网络被破坏时，如何重塑其蛋白质组。蛋白质组学数据可以提供有关细胞蛋白质库的广泛信息，以建立对细胞“身份”的客观理解。例如，了解代谢酶、营养转运蛋白、核糖体、翻译增强子和抑制子的丰度，可以评估不同淋巴细胞群体的代谢能力。为了利用蛋白质组数据集的价值，一个易于查询的在线资源至关重要，可以快速探索关键免疫细胞的蛋白质景观。有一个资源集中在外周血来源的人白细胞的子集5;然而，目前没有小鼠等效存在。因此，我们开发了ImmPRes作为一种资源，
Immunological Proteome Resource (ImmPRes; http://immpres. co. uk/). ImmPRes aims to provide an in-depth high-quality map of the immune cell proteomes. It is an initiative inspired by resources such as ImmGen 1, in which systematic analyses of RNA expression in immune cells have yielded invaluable biological insights about lymphocyte populations. ImmPRes maps the proteomes of immune cells rather than the transcriptome, which is important because changes in rates of protein synthesis and degradation mean that mRNA levels are not always effective predictors of cellular protein abundance 2. There are thus clear examples of discordance between mRNA and protein abundance in T cells that reflect the ability of post-transcriptional mechanisms to control T cell proteomes 3. Because proteins are the molecules that structure cells and control almost all metabolic processes and regulatory mechanisms 4, there is enormous value in the characterization of cellular proteomes to understand lymphocyte identity. Fortunately, technological advances in high-resolution mass spectrometry have made it feasible to characterize cell proteomes quantitatively. The technology can be used to explore how immune cells remodel their proteomes when they respond to immune or environmental stimuli or when key signaling networks are disrupted. Proteomic data can provide extensive information about the cellular repertoires of proteins to create an objective understanding of cell ‘identity’. For example, knowledge of the abundance of metabolic enzymes, nutrient transporters, ribosomes, and translational enhancers and repressors enables the assessment of the metabolic capacity of different lymphocyte populations.To leverage value from proteomic datasets, an easily interrogated online resource that enables rapid exploration of the protein landscape of key immune cells is vital. There is a resource focused on subsets of peripheral blood-derived human leukocytes 5; however, no current mouse equivalent exists. We have therefore developed ImmPRes as a resource to integrate data derived from high-resolution