Mobility of the piggyBac transposon in embryos of the vectors of Dengue fever (Aedes albopictus) and La Crosse encephalitis (Ae. triseriatus).
Mobility of the piggyBac transposon in embryos of the vectors of Dengue fever (Aedes albopictus) and La Crosse encephalitis (Ae. triseriatus).
复制标题
PiggyBac 转座子在登革热（白纹伊蚊）和拉克罗斯脑炎（三色伊蚊）载体胚胎中的移动性。
DOI：
10.1007/s004380000388
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
FraserJr,MJ
中科院分区：
文献类型：
--
作者：
Lobo,N;Li,X;Hua-Van,A;FraserJr,MJ
The re-emergence of arboviral diseases such as Dengue Fever and La Crosse encephalitis is primarily due to the failure of insect vector control strategies. The development of a procedure capable of producing stable germ-line transformants in the insect vectors of these diseases would bridge the gap between gene expression systems being developed to curb vector transmission and the identification of important genes and regulatory sequences and their reintroduction back into the insect genome in the form of vector control strategies. The transposable elementpiggyBacis capable of transposition in a variety of insect species, and could serve as a versatile insect transformation vector. Using plasmid-based excision and transposition assays, we report that this short-ITR transposon undergoes precise, transposase-dependent excision and transposition in embryos ofAedes albopictusandAedes triseriatus, the vectors of Dengue fever and LaCrosse encephalitis, respectively. These assays allow us easily and rapidly to confirm and assess the potential utility ofpiggyBacas a gene transfer tool in a given species.piggyBacis an exceptionally mobile and versatile genetic transformation vector, comparable to other transposons currently in use for the transformation of insects. The mobility of thepiggyBacelement seen in bothAe. albopictusandAe. triseriatusis further evidence that it can be employed as a germ-line vector in important insect disease vectors.
