Fourier transform infrared imaging spectroscopy (FT-IRIS) of mineralization in bisphosphonate-treated oim/oim mice
Fourier transform infrared imaging spectroscopy (FT-IRIS) of mineralization in bisphosphonate-treated oim/oim mice
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DOI：
10.1007/s00223-002-1038-1
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发表时间：
2003-05-01
影响因子：
4.2
通讯作者：
Raggio, CL
中科院分区：
文献类型：
--
作者：
Camacho, NP;Carroll, P;Raggio, CL
傅里叶变换红外显微镜（FT-IRM）和成像光谱（FT-IRIS）越来越多地用于分析矿化组织的分子成分。这些技术的主要优点是能够以7 pm的空间分辨率同时对组织切片中的多个组分的数量和质量进行成像。在目前的研究中，FT-骨密度和FT-IRIS被用来表征骨形成骨不连（OI）的小鼠模型治疗后，与双膦酸盐阿仑膦酸盐（ALN）的骨矿化。该应用目前具有相关性，因为最近的研究表明，双膦酸盐治疗儿童OI具有很大的前景，但尚未确定双膦酸盐相关的骨质变化。生长中的oim/oim小鼠是中度至重度OI的模型，从6至14周龄用ALN（73 μ g ALN/kg/天，持续4周，随后26 μ g/kg/天，持续4周）或生理盐水治疗，并评价股骨皮质和干骺端骨的矿化。矿物（碳酸磷灰石）和基质相的红外振动进行了监测。计算存在的矿物和基质的相对量（min：基质）、存在于矿物中的碳酸盐的相对量（carb：min）和矿物相的结晶度。在未处理的oim/oim骨中，与继发性松质骨相比，原发性松质骨的最小基质更大，结晶度降低（表明成熟矿物质较少），最可能是由于存在钙化软骨。ALN处理后，次生海绵体中的oim/oim mm：matrix增加，但矿物结晶度没有改变。在皮质骨中，ALN治疗没有明显变化。这些数据表明，在该小鼠模型中，ALN治疗导致干骺端骨矿化增加，但不改善矿物质成熟度。
Fourier transform infrared microscopy (FT-IRM) and imaging spectroscopy (FT-IRIS) are increasingly used to analyze the molecular components of mineralized tissues. A primary advantage of these techniques is the capability to simultaneously image the quantity and quality of multiple components in histological sections at 7 pm spatial resolution. In the current study, FT-IRM and FT-IRIS were used to characterize bone mineralization in a mouse model of osteogenesis imperfecta (OI) after treatment with the bisphosphonate alendronate (ALN). This application is currently relevant since recent studies have demonstrated great promise for the treatment of children with OI with bisphosphonates, but have not identified bisphosphonate-associated bone quality changes. Growing oim/oim mice, a model of moderate-to-severe OI, were treated with ALN (73 mug ALN/kg/day for 4 weeks followed by 26 mug/kg/day for 4 weeks) or saline from 6 to 14 weeks of age, and mineralization was evaluated in femoral cortical and metaphyseal bone. Infrared vibrations of the mineral (a carbonated apatite) and the matrix phases were monitored. The relative amounts of mineral and matrix present (min:matrix), the relative amount of carbonate present in the mineral (carb:min), and the crystallinity of the mineral phase were calculated. In untreated oim/oim bone, the min:matrix was greater and the crystallinity was reduced (indicative of less mature mineral) in the primary versus the secondary spongiosa, most likely due to the presence of calcified cartilage. With ALN treatment, the oim/oim mm:matrix increased in the secondary spongiosa, but the mineral crystallinity was not changed. In the cortical bone, no changes were evident with ALN treatment. These data demonstrate that in this mouse model, ALN treatment results in increased metaphyseal bone mineralization, but does not improve mineral maturity.