Vasoactive intestinal peptide as a neural mediator of gastric relaxation.
Vasoactive intestinal peptide as a neural mediator of gastric relaxation.
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血管活性肠肽作为胃松弛的神经介质。
DOI：
10.1152/ajpgi.1985.248.1.g73
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Makhlouf,GM
中科院分区：
文献类型：
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作者：
Grider,JR;Cable,MB;Said,SI;Makhlouf,GM
两个主要的候选者，腺苷5 '-三磷酸（ATP）和血管活性肠肽（VIP），已被提出作为肠道神经肌肉接头的抑制性递质。我们已经使用了ATP的光亲和类似物，3 '-O-（4-苯甲酰基）苯甲酰基ATP或BzATP，其共价结合ATP受体，并在光和特异性高亲和力VIP抗血清存在下使其失活，以检查ATP和VIP对豚鼠胃底环形平滑肌神经诱导的舒张的贡献。VIP和ATP引起剂量依赖性舒张; ATP的作用等于其稳定的电子等排体，α，β-亚甲基ATP，并且对腺苷脱氨酶的降解具有抗性，表明ATP与嘌呤能P2受体的相互作用。VIP抗血清（最终稀释度1：120）选择性抑制VIP诱导的舒张作用，而ATP诱导的舒张作用则被光活化BzATP选择性抑制。电场诱导的弛豫（即，VIP抗血清仅抑制对神经元的刺激，而光活化的BzATP则无此作用。对神经诱导的舒张的抑制范围从最低频率的86%（P <0.01）到最高频率的34%（P <0.01）。最大场刺激引起的VIP释放从壁内神经元增加了11倍。结果强烈支持VIP作为胃舒张的神经介质。
Two main candidates, adenosine 5'-triphosphate (ATP) and vasoactive intestinal peptide (VIP), have been proposed as inhibitory transmitters at neuromuscular junctions in the gut. We have used a photoaffinity analogue of ATP, 3'-O-(4-benzoyl)benzoyl ATP or BzATP, that binds covalently to ATP receptors and inactivates them in the presence of light and a specific high-affinity VIP antiserum in order to examine the contributions of ATP and VIP to neurally induced relaxation in circular smooth muscle of the gastric fundus of the guinea pig. VIP and ATP caused dose-dependent relaxation; the effect of ATP was equal to that of its stable isostere, alpha, beta-methylene ATP, and was resistant to degradation by adenosine deaminase, indicating interaction of ATP with purinergic P2-receptors. Relaxation induced by VIP was selectively inhibited by VIP antiserum (final dilution 1:120), while that induced by ATP was selectively inhibited by photoactivated BzATP. Relaxation induced by electrical field (i.e., neural) stimulation was inhibited by VIP antiserum only; photoactivated BzATP had no effect. Inhibition of neurally induced relaxation ranged from 86% (P less than 0.01) at the lowest frequencies to 34% (P less than 0.01) at the highest frequencies. Maximal field stimulation caused an 11-fold increase in VIP release from intramural neurons. The results strongly favor VIP as the neural mediator of gastric relaxation.