THE ASPERGILLUS-NIDULANS FLUG GENE IS REQUIRED FOR PRODUCTION OF AN EXTRACELLULAR DEVELOPMENTAL SIGNAL AND IS RELATED TO PROKARYOTIC GLUTAMINE-SYNTHETASE .1.
THE ASPERGILLUS-NIDULANS FLUG GENE IS REQUIRED FOR PRODUCTION OF AN EXTRACELLULAR DEVELOPMENTAL SIGNAL AND IS RELATED TO PROKARYOTIC GLUTAMINE-SYNTHETASE .1.
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DOI：
10.1101/gad.8.6.641
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发表时间：
1994-03-15
影响因子：
10.5
通讯作者：
ADAMS, TH
中科院分区：
文献类型：
--
作者：
LEE, BN;ADAMS, TH
Nidulans AspergillusFlug基因的突变破坏了无性孢子形成的程序性诱导，并导致形成蓬松的菌落，其特征是未分化的棉状营养细胞团。我们发现，当Flug突变菌落生长在野生型菌落旁边时，即使用6000-8000道尔顿孔径的透析膜分离，Flug突变菌落的表型也会受到抑制。Flug编码一种胞质定位的约96,000道尔顿多肽，在营养生长和发育诱导后相对稳定的水平存在。序列分析表明，864位密码子的Flug开放阅读框预测的羧基末端436个氨基酸与GSI型原核谷氨酰胺合成酶有约28%的同源性。我们认为，Flug不太可能在谷氨酰胺的合成中发挥作用，而是作为一种与GSI相关的酶来合成细胞外信号，指导无性产孢子形成，或许还有其他方面的菌落生长。讨论了由绒毛突变体鉴定的其他基因与Flug之间的关系。
Mutations in the Aspergillus nidulans fluG gene disrupt the programmed induction of asexual sporulation and result in formation of fluffy colonies that are characterized by undifferentiated cotton-like masses of vegetative cells. We show that the fluG mutant phenotype is suppressed when fluG mutant colonies are grown next to wild-type colonies even if the two strains are separated by dialysis membrane with a 6000- to 8000-dalton pore size. fluG encodes a cytoplasmically localized approximately 96,000-dalton polypeptide that is present at relatively constant levels during vegetative growth and following developmental induction. Sequence analysis of fluG demonstrated that the carboxy-terminal 436 amino acids predicted by the 864-codon FluG open reading frame shares approximately 28% identity with GSI-type prokaryotic glutamine synthetases. We consider it unlikely that FluG functions in synthesis of glutamine but instead propose that FluG functions as a GSI-related enzyme in synthesizing an extracellular signal directing asexual sporulation and perhaps other aspects of colony growth. The relationships between fluG and other genes identified by fluffy mutants are discussed.