Stochastic effects in bacterial communication mediated by extracellular vesicles
Stochastic effects in bacterial communication mediated by extracellular vesicles
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细胞外囊泡介导的细菌通讯的随机效应
DOI：
10.1103/physreve.107.024409
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发表时间：
2023
影响因子：
2.4
通讯作者：
Boedicker, James Q.
中科院分区：
文献类型：
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作者：
Weaver, Brian P.;Haselwandter, Christoph A.;Boedicker, James Q.
群体感应（QS）允许细菌细胞感知局部细胞密度的变化，从而调节多细胞过程，包括生物膜形成、毒力调节和水平基因转移。传统上，QS被认为涉及细胞外信号分子在溶液中的自由交换，但最近的实验表明，对于某些细菌系统，相当一部分信号分子被包装并在细胞外囊泡中传递。信号分子在细胞外囊泡中的包装如何影响细胞沟通和协调多细胞行为的能力，在很大程度上仍然是未知的。我们提出了QS的随机反应-扩散模型，该模型考虑了自由扩散和囊泡相关信号分子的交换。我们发现，信号分子通过细胞外囊泡的传递放大了信号浓度的局部波动，这可以强烈影响细菌通信的动力学和空间范围。对于具有多个菌落的系统，细胞外囊泡为菌落之间的信号传递提供了另一种途径，并且可能在自由信号分子强烈降解的环境中对长距离信号交换至关重要。
Quorum sensing (QS) allows bacterial cells to sense changes in local cell density and, hence, to regulate multicellular processes, including biofilm formation, regulation of virulence, and horizontal gene transfer. While, traditionally, QS was thought to involve the exchange of extracellular signal molecules free in solution, recent experiments have shown that for some bacterial systems a substantial fraction of signal molecules are packaged and delivered in extracellular vesicles. How the packaging of signal molecules in extracellular vesicles influences the ability of cells to communicate and coordinate multicellular behaviors remains largely unknown. We present here a stochastic reaction-diffusion model of QS that accounts for the exchange of both freely diffusing and vesicle-associated signal molecules. We find that the delivery of signal molecules via extracellular vesicles amplifies local fluctuations in the signal concentration, which can strongly affect the dynamics and spatial range of bacterial communication. For systems with multiple bacterial colonies, extracellular vesicles provide an alternate pathway for signal transport between colonies, and may be crucial for long-distance signal exchange in environments with strong degradation of free signal molecules.