Comparative Analysis of the Rats' Gut Microbiota Composition in Animals with Different Ginsenosides Metabolizing Activity
Comparative Analysis of the Rats' Gut Microbiota Composition in Animals with Different Ginsenosides Metabolizing Activity
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不同人参皂苷代谢活性动物大鼠肠道菌群组成的比较分析
DOI：
10.1021/acs.jafc.6b04848
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发表时间：
2017-01-18
影响因子：
6.1
通讯作者：
Quan, Lin-Hu
中科院分区：
文献类型：
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作者：
Dong, Wei-Wei;Xuan, Fang-Ling;Quan, Lin-Hu
口服人参后，主要的人参皂苷在吸收到血液中之前被肠道微生物群代谢为去糖基化的人参皂苷。由于个体之间肠道微生物群的组成不同，代谢活动也有显著差异。我们从口服人参提取物的60只大鼠中选择6只低效代谢（LEM）大鼠和6只高效代谢（HEM）大鼠，并使用16 S rRNA基因的Illumina HiSeq测序分析其肠道微生物群组成。LEM组和HEM组之间的肠道微生物群的组分显著不同。在两个类群中，S24-7、碱枝菌科和Erpipelotrichaceae占据了HEM类群的大部分OTU，显著高于LEM类群。此外，我们还分离到了能够将Rb 1转化为Rd的动物双歧杆菌GM 1。结果表明，这些肠道细菌可能在人参的代谢过程中起主导作用。
Following oral intake of Panax ginseng, major ginsenosides are metabolized to deglycosylated ginsenosides by gut microbiota before absorption into the blood. As the composition of gut microbiota varies between individuals, metabolic activities are significantly different. We selected 6 rats with low efficiency metabblism (LEM) and 6 rats with high efficiency metabolism (HEM) from 60 rats following oral administration of Panax ginseng, extract, and analyzed.their gut microbiota composition using Illumina HiSeq sequencing of the 16S rRNA gene. The components of gut microbiota between the LEM and HEM groups were significantly different. Between the 2 groups, S24-7, Alcaligendceae, and Erpipelotrichaceae occupied most OTUs of the HEM group, which was notably higher than the LEM group. Furthermore, we isolated Bifidobacterium animalis GM1 that could convert the ginsenoside Rbl to Rd. The result implies that these specific Intestinal bacteria may dominate the metabolism of Panax ginseng.