Live imaging of companion cells and sieve elements in Arabidopsis leaves.
Live imaging of companion cells and sieve elements in Arabidopsis leaves.
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DOI：
10.1371/journal.pone.0118122
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Dinant S
中科院分区：
文献类型：
--
作者：
Cayla T;Batailler B;Le Hir R;Revers F;Anstead JA;Thompson GA;Grandjean O;Dinant S
韧皮部是一种复杂的组织，由高度特化的细胞组成，这些细胞具有独特的亚细胞结构和紧密的组织形式，在细胞分辨率下进行体内研究具有挑战性。我们利用共聚焦扫描激光显微镜以及伴胞和筛管分子中的亚细胞荧光标记，对拟南芥叶片中的韧皮部进行活体成像。这种方法为明确识别韧皮部细胞类型提供了一个简单的框架。它凸显了伴胞内细胞器网状网络的紧密性。相比之下，在筛管分子中，在细胞周边观察到与PP2 - A1:GFP、GFP:RTM1和RTM2:GFP标记相关的未知物体。韧皮部凝集素PP2 - A1:GFP标记存在于壁旁基质中。它的位置不同于P - 蛋白丝的位置，P - 蛋白丝可通过SEOR1:GFP和SEOR2:GFP观察到。PP2 - A1:GFP围绕着两种物体，其中一种被鉴定为线粒体。这个位置表明它嵌入在筛管分子夹中，筛管分子夹是一种特殊结构，可能将细胞器彼此固定或固定在筛管的质膜上。GFP:RTM1与一类较大的物体相关联，可能对应于质体。PP2 - A1:GFP在未成熟筛管分子的细胞质中可溶。其在分化过程中亚细胞定位的变化为监测这一过程提供了一个体内蓝图。通过这些伴胞和筛管分子标记获得的亚细胞特征可作为探索体内韧皮部细胞组织和动态的标志。
The phloem is a complex tissue composed of highly specialized cells with unique subcellular structures and a compact organization that is challenging to study in vivo at cellular resolution. We used confocal scanning laser microscopy and subcellular fluorescent markers in companion cells and sieve elements, for live imaging of the phloem in Arabidopsis leaves. This approach provided a simple framework for identifying phloem cell types unambiguously. It highlighted the compactness of the meshed network of organelles within companion cells. By contrast, within the sieve elements, unknown bodies were observed in association with the PP2-A1:GFP, GFP:RTM1 and RTM2:GFP markers at the cell periphery. The phloem lectin PP2-A1:GFP marker was found in the parietal ground matrix. Its location differed from that of the P-protein filaments, which were visualized with SEOR1:GFP and SEOR2:GFP. PP2-A1:GFP surrounded two types of bodies, one of which was identified as mitochondria. This location suggested that it was embedded within the sieve element clamps, specific structures that may fix the organelles to each another or to the plasma membrane in the sieve tubes. GFP:RTM1 was associated with a class of larger bodies, potentially corresponding to plastids. PP2-A1:GFP was soluble in the cytosol of immature sieve elements. The changes in its subcellular localization during differentiation provide an in vivo blueprint for monitoring this process. The subcellular features obtained with these companion cell and sieve element markers can be used as landmarks for exploring the organization and dynamics of phloem cells in vivo.
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影响因子：
2.9
作者：
Ehlers, K;Knoblauch, M;van Bel, AJE
通讯作者：
van Bel, AJE
影响因子：
11.6
作者：
Knoblauch, M;van Bel, AJE
通讯作者：
van Bel, AJE
影响因子：
7.4
作者：
Hafke, JB;van Amerongen, JK;van Bel, AJE
通讯作者：
van Bel, AJE
DOI：
10.1073/pnas.97.7.3718
发表时间：
2000-03-28
影响因子：
11.1
作者：
Cutler, SR;Ehrhardt, DW;Somerville, CR
通讯作者：
Somerville, CR
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF