Application of Celluclast 1.5L in apple pectin extraction.
Application of Celluclast 1.5L in apple pectin extraction.
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DOI：
10.1016/j.carbpol.2015.07.051
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发表时间：
2015-12
影响因子：
11.2
通讯作者：
A. Wikiera;M. Mika;A. Starzyńska-Janiszewska;B. Stodolak
中科院分区：
文献类型：
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作者：
A. Wikiera;M. Mika;A. Starzyńska-Janiszewska;B. Stodolak
用Celluclast 1.5L从苹果渣中提取果胶，每克原料中加入25、50和75 μl果胶。在获得的果胶，半乳糖醛酸（GalA）的含量，中性糖（NS）的配置文件，甲基化程度（DM）和乙酰化程度（DAc），分子量，蛋白质，灰分和多酚水平以及抗氧化和抗肿瘤活性进行了测定。酶制剂的最低剂量导致果胶分离的产率与酸处理相当（15.3%）。更高剂量的应用进一步导致聚合物回收率增加近4%。酶法分离的果胶的特征在于较大的分子量，并含有更多的GalA的更高的DM和DAc比聚合物用酸提取。其富含蛋白质和多酚类物质，具有不同的NS谱，具有较高的抗自由基活性，并能抑制Caco-2腺癌细胞的增殖和侵袭。
Pectins were extracted from apple pomace with Celluclast 1.5L at a dose of 25, 50 and 75 μl per 1 g of material. In obtained pectin, the galacturonic acid (GalA) content, the neutral sugars (NS) profile, the degree of methylation (DM) and acetylation (DAc), the molecular mass, protein, ash and polyphenol levels as well as antioxidant and antitumor activity were determined. The lowest dose of enzymatic preparation resulted in the yield of pectin isolation comparable with acidic treatment (15.3%). Application of higher dose caused further, almost 4% increase in polymer recovery. Enzymatically isolated pectin was characterised by larger molecular mass and contained more GalA of higher DM and DAc than polymer extracted with acid. It was also richer in protein and polyphenols, and had different NS profile, which resulted in higher antiradical activity as well as the ability to inhibit the proliferation and invasion of Caco-2 adenocarcinoma cells.