Effect of cold and hot compress on neutrophilic migration to the site of doxorubicin extravasation.
Effect of cold and hot compress on neutrophilic migration to the site of doxorubicin extravasation.
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冷热敷对中性粒细胞迁移至阿霉素外渗部位的影响。
DOI：
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发表时间：
2019
影响因子：
1.4
通讯作者：
M. Yamakawa
中科院分区：
文献类型：
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作者：
Natsuko Miura;N. Aung;M. Yamakawa
在目前的研究中，我们研究了热和冷按压对阿霉素外渗的影响的部分机制。我们将20μL阿霉素(DOX)(1μg/μL)注射到小鼠背部皮下，观察冷包(18-20℃)、热包(38-40℃)组和不处理组(对照组)从第1天到第14天的大体和组织学变化。免疫荧光和RT-PCR检测C5a受体(CD88)、白介素8受体(IL-8RA)和瞬时受体电位阳离子通道V亚家族成员1(TRPV1)。肉眼观察，寒组皮损面积明显小于对照组，热组皮损面积明显大于对照组。中性粒细胞计数热组明显高于冷组(3h)和对照组。冷组CD88和IL-8RA表达的炎性细胞数在几乎各时间点均显著低于其他组，而热组在以后各时间点均显著低于其他组。在第1、3、14天，热组表达TRPV1的神经束数目均多于冷组。逆转录-聚合酶链式反应检测到CD88、IL-8RA和TRPV1在冷组和热包组均有表达。因此，这些结果表明，用于DOX外渗的冷敷可能会减轻炎症。
In the present study, we investigated a part of the mechanism responsible for the effects of hot and cold compresses for extravasation of doxorubicin. We injected 20 μl of doxorubicin (DOX) (1 μg/μl) subcutaneously into the dorsal area in mice and observed the resulting skin lesions macroscopically and histologically from day 1 to day 14 thereafter in groups treated with a cold pack (18-20°C) and a hot pack (38-40°C) or left untreated (control). Immunofluorescence and RT-PCR for C5a receptor (CD88), interleukin-8 receptor (IL-8RA), and transient receptor potential cation channel subfamily V member 1 (TRPV1) were also performed. Macroscopic observation showed that the area of the skin lesion was significantly smaller in the cold group than in the control group, but was significantly larger in the hot group. The neutrophil count in the lesion was significantly higher in the hot group than in the cold (3 hrs) and control groups. The numbers of inflammatory cells expressing CD88 and IL-8RA were significantly lower in the cold group than that in the other groups at almost time points and in the hot groups at later time points, respectively. The number of nerve fascicles expressing TRPV1 was higher in the hot group than in the cold group on days 1, 3 and 14. mRNA for CD88, IL-8RA and TRPV1 was detectable by reverse transcription-polymerase chain reaction in both the cold and hot pack groups. Consequently, these results suggested that the cold pack for the extravasation of DOX might reduce inflammation.
脓毒症中 C5a 受体表达增加。
DOI：
10.1172/jci15409
发表时间：
2002
期刊：
The Journal of clinical investigation.
影响因子：
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作者：
Riedemann,NielsC;Guo,Ren-Feng;Neff,ThomasA;Laudes,InesJ;Keller,KatieA;Sarma,VidyaJ;Markiewski,MaciejM;Mastellos,Dimitrios;Strey,ChristophW;Pierson,CarlL;Lambris,JohnD;Zetoune,FirasS;Ward,PeterA
通讯作者：
Ward,PeterA