γ-Aminobutyric acid production by culturable bacteria from the human intestine
γ-Aminobutyric acid production by culturable bacteria from the human intestine
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DOI：
10.1111/j.1365-2672.2012.05344.x
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发表时间：
2012-08-01
影响因子：
4
通讯作者：
Stanton, C.
中科院分区：
文献类型：
--
作者：
Barrett, E.;Ross, R. P.;Stanton, C.
目的评估人肠道来源的乳杆菌和双歧杆菌产生β-氨基丁酸(GABA)的能力。方法和结果乳杆菌和双歧杆菌在含有谷氨酸单钠(MSG)的培养基中培养。测定菌体生长和味精转化为GABA的能力。在被评估的91种肠道来源的细菌中，一种乳杆菌和四种双歧杆菌产生GABA。短链乳杆菌DPC6108是测试菌株中最有效的，它能将100%的味精转化为GABA。用简单的pH控制的厌氧粪便发酵，添加30个类似于mg的类似于ml-1的味精，研究了培养的肠道菌株产生GABA的能力。在粪便发酵中添加类似于Brivis DPC6108的乳酸菌显著提高了GABA浓度(P类似于
Aims To assess the ability of human intestinally derived strains of Lactobacillus and Bifidobacterium to produce ?-aminobutyric acid (GABA). Methods and Results Strains of Lactobacillus and Bifidobacterium were grown in medium containing monosodium glutamate (MSG). Growth of the bacteria and conversion of MSG to GABA were measured. Of 91 intestinally derived bacteria assessed, one Lactobacillus strain and four strains of Bifidobacterium produced GABA. Lactobacillus brevis DPC6108 was the most efficient of the strains tested, converting up to 100% of MSG to GABA. The ability of the cultured intestinal strains to produce GABA was investigated using a simple pH-controlled anaerobic faeces-based fermentation, supplemented with 30 similar to mg similar to ml-1 MSG. The addition of Lact.similar to brevis DPC6108 to a faeces-based fermentation significantly increased the GABA concentration (P similar to