Cloned cDNA to cholecystokinin mRNA predicts an identical preprocholecystokinin in pig brain and gut.
Cloned cDNA to cholecystokinin mRNA predicts an identical preprocholecystokinin in pig brain and gut.
复制标题
胆囊收缩素 mRNA 的克隆 cDNA 预测猪脑和肠道中存在相同的前胆囊收缩素。
DOI：
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发表时间：
1984
影响因子：
11.1
通讯作者：
J. Eng
中科院分区：
文献类型：
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作者：
U. Gubler;A. O. Chua;B. Hoffman;K. Collier;J. Eng
分子克隆已在猪大脑皮层和十二指肠粘膜中建立了前胆囊收缩素原的结构。这种前体长114个氨基酸，在脑和肠中是相同的，包含先前分离的所有胆囊收缩素（CCK）肽，并具有激素原的特征。它含有一个假定的氨基末端信号肽、碱性加工位点和羧基末端酰胺化信号。来自大脑和肠道的CCK mRNA长约850个核苷酸，仅存在几个单碱基变化。这种分析建立了一个严格的标准，CCK是在大脑和肠道中合成的，CCK的分子形式在两种组织中的不同分布最有可能是组织特异性翻译后事件的结果。
Molecular cloning has established the structure of preprocholecystokinin in porcine cerebral cortex and duodenal mucosa. This precursor is 114 amino acids long, is identical in brain and gut, contains all the cholecystokinin (CCK) peptides previously isolated, and has the characteristics of a prohormone. It contains a putative amino-terminal signal peptide, basic processing sites, and a carboxyl-terminal amidation signal. The CCK mRNAs from brain and gut are approximately 850 nucleotides long and differ by only a few single base changes. This analysis establishes by a strict criterion that CCK is synthesized in both brain and gut and that the different distributions of molecular forms of CCK in the two tissues are most probably a consequence of tissue-specific posttranslational events.