Increased cucumber salt tolerance by grafting on pumpkin rootstock and after application of calcium
Increased cucumber salt tolerance by grafting on pumpkin rootstock and after application of calcium
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DOI：
10.1007/s10535-013-0349-6
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发表时间：
2014-03
影响因子：
1.5
通讯作者：
B. Lei;Y. Huang;J. J. Xie-J.;Z. X. Liu;A. Zhen;M. Fan;Z. Bie
中科院分区：
文献类型：
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作者：
B. Lei;Y. Huang;J. J. Xie-J.;Z. X. Liu;A. Zhen;M. Fan;Z. Bie
以黄瓜自接和南瓜砧木嫁接植株为材料，进行4种营养液处理：1）单独通气营养液（对照），2）10 mM Ca（NO3）2（Ca）营养液，3）90 mM NaCl（NaCl）营养液，4）90 mM NaCl + 10 mM Ca（NO3）2（NaCl+Ca）营养液。NaCl处理降低了植株干物质质量和Ca ~（2+）、K ~+含量，但增加了根和地上部Na ~+含量。在南瓜砧木嫁接植物中观察到较小的变化。Ca（NO3）2的添加缓解了NaCl对南瓜干物质积累、相对生长速率（RGR）以及Ca 2+、K+和Na+含量的负面影响，尤其是对南瓜砧木嫁接植株。补充Ca（NO3）2可明显刺激质膜H ~+-ATPase活性，为Na ~+的清除提供能量。外源Ca（NO3）2可上调质膜H+-ATP酶基因（PMA）和质膜Na+/H+逆向转运蛋白基因（SOS 1）的表达。在NaCl + Ca处理下，嫁接植株的PM H+-ATPase活性、PMA和SOS 1表达量均高于自接植株。因此，外源Ca 2+与南瓜砧木嫁接相结合是提高黄瓜耐盐性的有效途径。
Self-grafted and pumpkin rootstock-grafted cucumber plants were subjected to the following four treatments:1) aerated nutrient solution alone (control),2) nutrient solution with 10 mM Ca(NO3)2(Ca),3) nutrient solution with 90 mM NaCl (NaCl), and4) nutrient solution with 90 mM NaCl + 10 mM Ca(NO3)2(NaCl+Ca). The NaCl treatment decreased the plant dry mass and content of Ca2+and K+but increased the Na+content in roots and shoots. Smaller changes were observed in pumpkin rootstock-grafted plants. Supplementary Ca(NO3)2ameliorated the negative effects of NaCl on plant dry mass, relative growth rate (RGR), as well as Ca2+, K+, and Na+content especially for pumpkin rootstock-grafted plants. Supplementary Ca(NO3)2distinctly stimulated the plasma membrane (PM) H+-ATPase activity which supplies the energy to remove excess Na+from the cells. The expressions of gene encoding PM H+-ATPases (PMA) and gene encoding a PM Na+/H+antiporter (SOS1) were up-regulated when Ca(NO3)2was applied. The pumpkin rootstock-grafted plants had higher PM H+-ATPase activity as well as higherPMAandSOS1expressions than the self-grafted plants under NaCl + Ca treatment. Therefore, the addition of Ca2+in combination with pumpkin rootstock grafting is a powerful way to increase cucumber salt tolerance.