Genomic analysis of highly virulent Georgia 2007/1 isolate of African swine fever virus.
Genomic analysis of highly virulent Georgia 2007/1 isolate of African swine fever virus.
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DOI：
10.3201/eid1704.101283
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发表时间：
2011-04
影响因子：
11.8
通讯作者：
Dixon LK
中科院分区：
文献类型：
--
作者：
Chapman DA;Darby AC;Da Silva M;Upton C;Radford AD;Dixon LK
序列信息将促进疫苗开发的研究。非洲猪瘟在非洲很普遍，但偶尔也会传入其他大陆。2007年6月，非洲猪瘟在格鲁吉亚共和国的高加索地区被隔离，随后在邻国（亚美尼亚、阿塞拜疆和俄罗斯联邦的9个州）被隔离。先前的3个基因测序数据表明，格鲁吉亚2007/1分离株与已在莫桑比克、马达加斯加和赞比亚鉴定的基因型II分离株密切相关。我们报告了格鲁吉亚2007/1分离株的完整基因组编码序列，并与其他分离株进行了比较。获得了189，344 bp的基因组序列，编码166个开放阅读框（ORF）。基于125个保守ORF的串联序列的系统发育表明，该分离物与Mkuzi 1979分离物最接近。一些ORF以不同的方式聚集，表明可能发生了重组。结果为监测该病毒的基因组变化提供了基线。
Sequence information will facilitate research on vaccine development. African swine fever is widespread in Africa but has occasionally been introduced into other continents. In June 2007, African swine fever was isolated in the Caucasus Region of the Republic of Georgia and subsequently in neighboring countries (Armenia, Azerbaijan, and 9 states of the Russian Federation). Previous data for sequencing of 3 genes indicated that the Georgia 2007/1 isolate is closely related to isolates of genotype II, which has been identified in Mozambique, Madagascar, and Zambia. We report the complete genomic coding sequence of the Georgia 2007/1 isolate and comparison with other isolates. A genome sequence of 189,344 bp encoding 166 open reading frames (ORFs) was obtained. Phylogeny based on concatenated sequences of 125 conserved ORFs showed that this isolate clustered most closely with the Mkuzi 1979 isolate. Some ORFs clustered differently, suggesting that recombination may have occurred. Results provide a baseline for monitoring genomic changes in this virus.
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