The Combination of Tissue Dissection and External Volume Expansion Generates Large Volumes of Adipose Tissue
The Combination of Tissue Dissection and External Volume Expansion Generates Large Volumes of Adipose Tissue
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组织解剖和外部体积扩张相结合产生大量脂肪组织
DOI：
10.1097/prs.0000000000003212
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发表时间：
2017-04-01
影响因子：
3.6
通讯作者：
Lu, Feng
中科院分区：
文献类型：
--
作者：
He, Yunfan;Dong, Ziqing;Lu, Feng
背景：非侵入性外扩容装置已在临床上应用于刺激非手术乳房增大。尽管先前的研究结果表明，外部体积扩张能够增加脂肪细胞的数量，但仅靠这一策略不足以重建软组织缺陷或增加乳房质量。作者将微创组织剥离方法与外部体积扩张相结合，产生了大量脂肪组织。方法：体外制备不同密度的脂肪干细胞，评价细胞接触与细胞增殖的关系。体内解剖兔背部脂肪组织，应用体外扩容装置维持释放状态。以不进行组织剥离的体外扩容为对照。结果：解剖组各时间点脂肪组织生成量均明显大于对照组。在组织剥离后的早期阶段，解剖标本中出现的增殖细胞数量明显多于对照标本。在低细胞密度下，脂肪干细胞表现出比高细胞密度更高的增殖率。蛋白质表达分析表明，细胞增殖在体内和体外都是由相似的机制介导的，包括细胞接触抑制的释放和Hippo/Yes相关蛋白通路的激活。结论：脂肪组织剥离可释放细胞间的接触，并诱导脂肪干细胞增殖。预扩增的脂肪干细胞在体外体积扩张的成脂环境下进行成脂，与对照组相比脂肪再生效果更好。
Background: Noninvasive external volume expansion device has been applied to stimulate nonsurgical breast enlargement in clinical settings. Although previous results demonstrate the capacity of external volume expansion to increase the number of adipocytes, this strategy alone is insufficient to reconstruct soft-tissue defects or increase breast mass. The authors combined a minimally invasive tissue dissection method with external volume expansion to generate large volumes of adipose tissue. Method: In vitro, various densities of adipose-derived stem cells were prepared to evaluate relations between cell contacts and cell proliferation. In vivo, dorsal adipose tissue of rabbits was thoroughly dissected and the external volume expansion device was applied to maintain the released state. External volume expansion without tissue dissection served as the control. Results: In the dissection group, the generated adipose tissue volume was much larger than that in the control group at all time points. A larger number of proliferating cells appeared in the dissection samples than in the control samples at the early stage after tissue dissection. At low cell density, adipose-derived stem cells displayed an increasing proliferation rate compared to high cell density. Protein expression analysis revealed that cell proliferation was mediated by a similar mechanism both in vivo and in vitro, involving the release of cell contact inhibition and Hippo/Yes-associated protein pathway activation. Conclusions: Adipose tissue dissection releases cell-to-cell contacts and induces adipose-derived stem cell proliferation. Preexpanded adipose-derived stem cells undergo adipogenesis under the adipogenic environment created by external volume expansion, leading to better adipose regeneration compared with the control.