Hypothalamic gonadotrophin-releasing hormone expression in female monkeys with different sensitivity to stress
Hypothalamic gonadotrophin-releasing hormone expression in female monkeys with different sensitivity to stress
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DOI：
10.1111/j.1365-2826.2007.01566.x
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发表时间：
2007-08-01
影响因子：
3.2
通讯作者：
Bethea, C. L.
中科院分区：
文献类型：
--
作者：
Centeno, M.-L.;Sanchez, R. L.;Bethea, C. L.
在寻求下丘脑性闭经治疗的妇女中观察到心理社会压力，加上轻度节食和适度运动。使用雌性食蟹猴，我们以前报告说，相同的组合温和的压力抑制生殖激素分泌和月经周期在一些人（压力敏感，SS），但不是在其他人（高压力弹性，HSR）。与HSR猴相比，SS猴在非应激周期的中期峰值表现出较低的雌二醇和孕酮水平，并且在中枢孕酮能系统中的基因表达降低。由于类固醇和5-羟色胺影响下丘脑-垂体-性腺（HPG）轴，我们假设SS和HSR猴之间的差异，在HPG轴的敏感性，以压力可能最终表现在促性腺激素释放激素（GnRH）系统的差异。GnRH原位杂交和免疫组织化学进行了与SS和HSR动物的下丘脑切片，安乐死在一个非应激月经周期的早期卵泡期。与HSR猴相比，SS猴的GnRH细胞体的数量和密度显著更高，以及具有非常强的GnRH mRNA表达的索马体的数量更高，但SS猴的正中隆起中免疫染色的GnRH纤维的密度更低。我们认为，参与控制GnRH的合成，运输和释放的神经元机制不同SS相比，HSR动物。
Psychosocial stress, combined with mild dieting and moderate exercise, are observed in women seeking treatment for hypothalamic amenorrhea. Using female cynomolgus macaques, we previously reported that the same combination of mild stresses suppressed reproductive hormone secretion and menstrual cycles in some individuals (stress-sensitive, SS), but not in others (highly stress-resilient, HSR). Compared to HSR monkeys, SS monkeys exhibited lower oestradiol and progesterone levels at the midcycle peak and decreased gene expression in the central serotonergic system during nonstressed cycles. Because steroids and serotonin impinge upon the hypothalamic-pituitary-gonadal (HPG) axis, we hypothesised that the differences between SS and HSR monkeys in the sensitivity of the HPG axis to stress may ultimately manifest in differences in the gonadotrophin-releasing hormone (GnRH) system. GnRH in situ hybridisation and immunohistochemistry were performed with hypothalamic sections from SS and HSR animals, euthanised in the early follicular phase of a nonstressed menstrual cycle. Compared to HSR monkeys, SS monkeys exhibited a significantly higher number and density of GnRH cell bodies, as well as a higher number of soma with extremely robust expression of GnRH mRNA, but SS monkeys exhibited a lower density of immunostained GnRH fibres in the median eminence. We suggest that neuronal mechanisms involved in the control of GnRH synthesis, transport and release differ in SS compared to HSR animals.