Effect of Chloramphenicol on Host-Bacteriophage Relationships in the Lactic Streptococci
Effect of Chloramphenicol on Host-Bacteriophage Relationships in the Lactic Streptococci
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氯霉素对乳酸链球菌宿主-噬菌体关系的影响
DOI：
10.1128/am.19.5.707-713.1970
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
J. Erskine
中科院分区：
文献类型：
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作者：
J. Erskine
氯霉素（CM）的乳酸链球菌菌株ML 3的耐药突变体获得的结果，连续转移的细菌在肉汤中含有越来越多的CM或通过选择高水平的耐药衍生物后，诱变处理的细菌。用第一种方法获得的一些CM抗性细胞也对同源噬菌体具有抗性。在CM存在下培养生长的细胞对一些异源攻击性噬菌体产生了抗性，但对噬菌体ml 3没有抗性。针对噬菌体抗性选择的突变体对CM没有抗性。在细菌染色体上似乎有两个不同的CM抗性基因座：高水平抗性的一个与噬菌体抗性位点相关，另一个与噬菌体抗性位点无关。（280 μg/ml）抑制菌株ML 3-CMrI中ml 3噬菌体的细胞内生长，所述菌株ML 3-CMrI已被训练成在该CM浓度存在下生长，尽管该菌株的细胞本身不是噬菌体抗性的。该药物没有直接的杀病毒作用，也不能阻止噬菌体吸附或渗透到细菌中。未发生溶源化。它的结论是，在噬菌体发展的块可能涉及抑制噬菌体成分的合成，无论是涉及脱氧核糖核酸在早期阶段或噬菌体外壳蛋白在稍后的。
Chloramphenicol (CM)-resistant mutants of Streptococcus lactis strain ML3 were obtained either as a consequence of continuous transfer of the bacteria in broth containing increasing amounts of CM or by selecting for high-level resistant derivatives after mutagenic treatment of the bacteria. Some CM-resistant cells obtained by the first method were also resistant to the homologous bacteriophage. Cells trained to grow in the presence of CM developed resistance to some heterologous attacking phages but not to phage ml3. Mutants selected for phage resistance were not resistant to CM. There appear to be two different loci for CM resistance on the bacterial chromosome: the one for high-level resistance is associated with the phage-resistance locus and the other is independent of it. A concentration of CM (280 μg/ml) that was bacteriostatic for ML3 inhibited the intracellular growth of ml3 phage in strain ML3-CMrI, which had been trained to grow in the presence of that CM concentration, despite the fact that cells of this strain were not phage-resistant per se. The drug had no direct virucidal action and did not prevent adsorption to or penetration of phage into the bacterium. Lysogenization did not occur. It is concluded that the block in phage development probably involves inhibition of synthesis of phage components, either involving deoxyribonucleic acid at an early stage or the phage coat protein at a later one.