Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates
Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates
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DOI：
10.1016/s0968-0896(02)00204-3
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发表时间：
2002-10-01
影响因子：
3.5
通讯作者：
Dervan, PB
中科院分区：
文献类型：
--
作者：
Belitsky, JM;Leslie, SJ;Dervan, PB
用激光共聚焦扫描显微镜研究了DNA结合的N-甲基吡咯/N-甲基咪唑聚酰胺-染料结合物在多种活细胞中的摄取和定位特性。除了某些T细胞系外，聚酰胺-染料结合物主要定位于细胞质，而不是细胞核。通常用于固定显微镜细胞的试剂，如甲醇，极大地改变了这些DNA结合配体的细胞定位。(C)2002爱思唯尔科学有限公司。保留所有权利。
The cellular uptake and localization properties of DNA binding N-methylpyrrole/N-methylimidazole polyamide-dye conjugates in a variety of living cells have been examined by confocal laser scanning microscopy. With the exception of certain T-cell lines, polyamide-dye conjugates localize mainly in the cytoplasm and not in the nucleus. Reagents such as methanol typically used to fix cells for microscopy significantly alter the cellular localization of these DNA-binding ligands. (C) 2002 Elsevier Science Ltd. All rights reserved.