Messenger ribonucleic acid levels in disrupted human anterior cruciate ligaments
Messenger ribonucleic acid levels in disrupted human anterior cruciate ligaments
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DOI：
10.1097/01.blo.0000037442.23683.35
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发表时间：
2003-02-01
影响因子：
4.2
通讯作者：
Frank, CB
中科院分区：
文献类型：
--
作者：
Lo, IKY;Marchuk, L;Frank, CB
30例患者因持续性不稳定接受前交叉韧带重建。根据重建过程中确定的大体形态学特征定义了两组：前交叉韧带断裂伴瘢痕附着于关节结构和断裂无再附着。对重建手术中切除的瘢痕前交叉韧带组织样本进行细胞外基质分子、蛋白酶和蛋白酶抑制剂亚类的逆转录聚合酶链反应。结果显示，非附着瘢痕组的I型胶原的mRNA水平显着增加，和增加的I型到III型胶原的比例相比，附着瘢痕组。在损伤后的第一年，非附着性瘢痕组的核心蛋白聚糖mRNA水平也显著高于附着性瘢痕组。非附着性瘢痕组中双糖蛋白聚糖mRNA水平与I型胶原mRNA水平密切相关。这些结果表明，在撕裂的前交叉韧带的细胞表达的差异，连接到结构的关节与那些不。尽管导致这些差异的分子机制尚未阐明，但不同的分子信号可能会影响前交叉韧带断裂的大体形态学特征，或者不同的大体形态学特征可能会受到不同的机械负荷，导致分子表达改变。然而，在损伤的前交叉韧带中发现内源性细胞活性提高了增强这种活性以改善结果的可能性。
Thirty patients had anterior cruciate ligament reconstruction for ongoing instability. Two groups were defined according to gross morphologic features identified during reconstruction: anterior cruciate ligament disruptions with scars attached to a structure in the joint and disruptions without reattachments. Reverse transcription polymerase chain reaction for a subset of extracellular matrix molecules, proteinases, and proteinase inhibitors was done on samples of scarred anterior cruciate ligament tissue removed during reconstructive surgery. Results of the nonattached scar group showed significantly increased mRNA levels for Type I collagen, and an increased Type I to Type III collagen ratio compared with that for the attached scar group. In the first year after injury, decorin mRNA levels in the nonattached scar group also were significantly higher than in the attached scar group. Biglycan mRNA levels in the nonattached scar group correlated closely with Type I collagen mRNA levels. These results suggest differences in cellular expression in torn anterior cruciate ligaments that attach to structures in the joint versus those which do not. Although the molecular mechanisms responsible for these differences have not been delineated, different molecular signals may influence the gross morphologic features of anterior cruciate ligament disruptions or alternatively, differing gross morphologic features may be subject to different mechanical loads leading to altered molecular expression. However, the finding of endogenous cellular activity in injured anterior cruciate ligaments raises the possibility that this activity may be enhanced to improve outcomes.