Isolation and characterization of three DREB/ERF-type transcription factors from melon (Cucumis melo)
Isolation and characterization of three DREB/ERF-type transcription factors from melon (Cucumis melo)
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甜瓜（Cucumis melo）中三种 DREB/ERF 型转录因子的分离和表征
DOI：
10.1016/j.plantsci.2006.02.005
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发表时间：
2006-06-01
期刊：
影响因子：
5.2
通讯作者：
Sato, Takahide
中科院分区：
文献类型：
--
作者：
Mizuno, Shinji;Hirasawa, Yosuke;Sato, Takahide
甜瓜1-氨基环丙烷-1-羧酸合酶（CMe-ACS2）是甜瓜乙烯生物合成途径中的关键酶，但调节CMe-ACS2表达的机制仍有待阐明。我们鉴定了三种甜瓜 AP2/ERF 转录因子（CMe-DREB1、CMe-ERF1 和 CMe-ERF2），它们是通过酵母单杂交筛选方法使用 CMe-ACS2 启动子中的 GCCGAC 序列（DRE/CRT 顺式作用元件）分离的。我们证明CMe-DREB1与GCCGAC序列强烈结合，但CMe-ERF1和CMe-ERF2没有。瞬时表达分析表明，只有CMe-DREB1能够在瓜叶细胞中通过GCCGAC序列激活下游报告基因的转录。茉莉酸甲酯（MeJA）处理增加了瓜叶中CMe-ACS2和CMe-DREB1的表达水平。这些结果表明，在茉莉酸存在的情况下，CMe-DREB1 作为 CMe-ACS2 的转录激活剂发挥作用。 (c) 2006 Elsevier Ireland Ltd. 保留所有权利。
A melon 1-aminocyclopropane-1-carboxylate synthase (CMe-ACS2) is a key enzyme in the ethylene biosynthesis pathway in melon, but mechanisms regulating CMe-ACS2 expression remain to be clarified. We characterized three melon AP2/ERF transcription factors (CMe-DREB1, CMe-ERF1 and CMe-ERF2), which were isolated by a yeast one-hybrid screening method using a GCCGAC sequence (DRE/CRT cis-acting element) in the CMe-ACS2 promoter. We demonstrated that CMe-DREB1 strongly bound to the GCCGAC sequence, but CMe-ERF1 and CMe-ERF2 did not. Transient expression analysis showed that only CMe-DREB1 could activate the transcription of the downstream reporter gene through the GCCGAC sequence in melon leaf cells. The expression levels of CMe-ACS2 and CMe-DREB1 in melon leaves were increased by methyljasmonate (MeJA) treatment. These results suggest that CMe-DREB1 functions as a transcriptional activator of CMe-ACS2 in the presence of jasmonate. (c) 2006 Elsevier Ireland Ltd. All rights reserved.