Assembly of a parts list of the human mitotic cell cycle machinery
Assembly of a parts list of the human mitotic cell cycle machinery
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DOI：
10.1093/jmcb/mjy063
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发表时间：
2019-08-01
影响因子：
5.5
通讯作者：
Freeman, Tom C.
中科院分区：
文献类型：
--
作者：
Giotti, Bruno;Chen, Sz-Hau;Freeman, Tom C.
有丝分裂所需的一组蛋白质仍然缺乏特点。在此，对人类和小鼠转录组学数据进行了广泛的一系列相关性分析，以鉴定与经历细胞周期S/G2-M期的细胞强烈且可重复相关的基因。通过这样做，确定了701个细胞周期相关基因，虽然显示许多基因仅在这些阶段表达，但其他基因的表达也由替代启动子驱动。在这个列表中，496个基因具有已知的细胞周期功能，而205个被指定为推定的细胞周期基因，其中53个是功能不明的。其中，27个被筛选的亚细胞定位揭示了许多核定位和至少三个是新的中心体蛋白。此外，10个其他siRNA敲除抑制细胞增殖。这项研究提出了人类细胞周期蛋白的第一个全面列表，确定了许多新的候选蛋白。
The set of proteins required for mitotic division remains poorly characterized. Here, an extensive series of correlation analyses of human and mouse transcriptomics data were performed to identify genes strongly and reproducibly associated with cells undergoing S/G2-M phases of the cell cycle. In so doing, 701 cell cycle-associated genes were defined and while it was shown that many are only expressed during these phases, the expression of others is also driven by alternative promoters. Of this list, 496 genes have known cell cycle functions, whereas 205 were assigned as putative cell cycle genes, 53 of which are functionally uncharacterized. Among these, 27 were screened for subcellular localization revealing many to be nuclear localized and at least three to be novel centrosomal proteins. Furthermore, 10 others inhibited cell proliferation upon siRNA knockdown. This study presents the first comprehensive list of human cell cycle proteins, identifying many new candidate proteins.