Bacterial Vaginosis Is Associated with Loss of Gamma Delta T Cells in the Female Reproductive Tract in Women in the Miami Women Interagency HIV Study (WIHS): A Cross Sectional Study.
Bacterial Vaginosis Is Associated with Loss of Gamma Delta T Cells in the Female Reproductive Tract in Women in the Miami Women Interagency HIV Study (WIHS): A Cross Sectional Study.
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DOI：
10.1371/journal.pone.0153045
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Fischl MA
中科院分区：
文献类型：
--
作者：
Alcaide ML;Strbo N;Romero L;Jones DL;Rodriguez VJ;Arheart K;Martinez O;Bolivar H;Podack ER;Fischl MA
细菌性阴道病（BV）是最常见的女性生殖道感染，它与感染和传播艾滋病毒的风险增加有关，其机制尚不清楚。 γ δ (GD) T 细胞是适应性和先天免疫系统的重要组成部分，存在于女性生殖道中，在上皮屏障保护中发挥重要作用。 GD1 细胞在粘膜组织中占主导地位，对于维持粘膜完整性很重要。 GD2细胞在外周血中占主导地位，在体液免疫和对病原体的免疫反应中发挥作用。 HIV 感染与外周和女性生殖道中 GD T 细胞频率的变化有关。本研究的目的是评估 BV 引起的阴道菌群变化是否与宫颈内 GD T 细胞反应的变化有关，这可能是 HIV 易感性增加的原因。 17 名 HIV 感染者 (HIV+) 和 17 名 HIV 未感染者 (HIV-) 绝经前妇女接受了阴道拭子和宫颈细胞刷的采集。使用 Nugent 评分评估阴道菌群。通过流式细胞术评估细胞刷样品中的 GD T 细胞。 Nugent 评分中位数为 5.0，41% 的女性阴道菌群异常。在未感染 HIV 的女性中，Nugent 评分与宫颈 GD1 T 细胞之间呈负相关（相互作用的 b = - 0.176，p<0.01）；阴道菌群正常的女性的宫颈 GD1 T 细胞含量高于菌群异常的女性（45.00% vs 9.95%，p = 0.005）；菌群异常的女性的宫颈 GD2 T 细胞含量高于菌群正常的女性（1.70% vs 0.35%，p = 0.023）。生殖道中的 GD T 细胞具有保护作用 (GD1)，并且是 HIV 进入的目标 (GD2)。阴道菌群异常的女性中，宫颈 GD1 的减少和 GD2 T 细胞的增加使患有 BV 的女性更容易感染 HIV。我们建议使用 GD T 细胞作为女性生殖道艾滋病毒易感性的标记。
Bacterial vaginosis (BV) is the most common female reproductive tract infection and is associated with an increased risk of acquiring and transmitting HIV by a mechanism that is not well understood. Gamma delta (GD) T cells are essential components of the adaptive and innate immune system, are present in the female reproductive tract, and play an important role in epithelial barrier protection. GD1 cells predominate in the mucosal tissue and are important in maintaining mucosal integrity. GD2 cells predominate in peripheral blood and play a role in humoral immunity and in the immune response to pathogens. HIV infection is associated with changes in GD T cells frequencies in the periphery and in the female reproductive tract. The objective of this study is to evaluate if changes in vaginal flora occurring with BV are associated with changes in endocervical GD T cell responses, which could account for increased susceptibility to HIV. Seventeen HIV-infected (HIV+) and 17 HIV-uninfected (HIV-) pre-menopausal women underwent collection of vaginal swabs and endocervical cytobrushes. Vaginal flora was assessed using the Nugent score. GD T cells were assessed in cytobrush samples by flow cytometry. Median Nugent score was 5.0 and 41% of women had abnormal vaginal flora. In HIV uninfected women there was a negative correlation between Nugent score and cervical GD1 T cells (b for interaction = - 0.176, p<0.01); cervical GD1 T cells were higher in women with normal vaginal flora than in those with abnormal flora (45.00% vs 9.95%, p = 0.005); and cervical GD2 T cells were higher in women with abnormal flora than in those with normal flora (1.70% vs 0.35%, p = 0.023). GD T cells in the genital tract are protective (GD1) and are targets for HIV entry (GD2). The decrease in cervical GD1 and increase in GD2 T cells among women with abnormal vaginal flora predisposes women with BV to HIV acquisition. We propose to use GD T cell as markers of female genital tract vulnerability to HIV.