Expression analysis of the NDRG2 gene in mouse embryonic and adult tissues
Expression analysis of the NDRG2 gene in mouse embryonic and adult tissues
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DOI：
10.1007/s00441-005-0137-5
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发表时间：
2006-07-01
影响因子：
3.6
通讯作者：
Yao, Li-Bo
中科院分区：
文献类型：
--
作者：
Hu, Xiao-Lan;Liu, Xin-Ping;Yao, Li-Bo
N-myc 下游调节基因 2 (NDRG2) 被认为与细胞生长事件有关。然而，其确切功能仍然未知。为了阐明该基因的作用，我们在免疫组织化学和免疫荧光测定中使用抗Ndrg2单克隆抗体来分析Ndrg2蛋白在不同胎龄的小鼠胚胎和多种成年小鼠组织中的表达模式。 Ndrg2 免疫反应性通常位于细胞质。在小鼠发育过程中，在许多发育中的组织和器官中观察到 Ndrg2 表达，包括心脏、大脑、肺、肠道、肝脏、肾脏、骨骼肌、软骨、绒毛膜、表皮和胡须毛囊。 Ndrg2 表达具有发育动态性，在发育早期阶段通常较低，在后期阶段显着增加。在多种成年小鼠组织中也观察到 Ndrg2 表达，特别是在心脏和大脑中。这是 Ndrg2 蛋白在胚胎和成年小鼠组织中表达的首次证明。我们的结果表明 NDRG2 在组织发生和器官发生中发挥重要作用。
N-myc downstream-regulated gene 2 (NDRG2) is believed to be involved in cell growth events. However, its exact function is still unknown. To elucidate the role of this gene, we used an anti-Ndrg2 monoclonal antibody in immunohistochemistry and immunofluorescence assays to analyze the expression pattern of Ndrg2 protein in mouse embryos at various gestational ages and in a variety of adult mouse tissues. Ndrg2 immunoreactivity was generally localized to the cytoplasm. During mouse development, Ndrg2 expression was observed in many developing tissues and organs including the heart, brain, lung, gut, liver, kidney, skeletal muscle, cartilage, chorion, epidermis, and whisker follicles. Ndrg2 expression was developmentally dynamic, being generally lower in the early stages of development and markedly increasing during later stages. Ndrg2 expression was also observed in a variety of adult mouse tissues, particularly in the heart and brain. This is the first demonstration of Ndrg2 protein expression in both embryonic and adult mouse tissues. Our results suggest that NDRG2 plays important roles in histogenesis and organogenesis.