A comprehensive DNA barcode database for Central European beetles with a focus on Germany: adding more than 3500 identified species to BOLD
A comprehensive DNA barcode database for Central European beetles with a focus on Germany: adding more than 3500 identified species to BOLD
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DOI：
10.1111/1755-0998.12354
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发表时间：
2015-07-01
影响因子：
7.7
通讯作者：
Balke, Michael
中科院分区：
文献类型：
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作者：
Hendrich, Lars;Moriniere, Jerome;Balke, Michael
甲虫是最多样化的动物群体，对生态系统功能至关重要。在许多国家，它们已被很好地用于环境影响评估，但即使是在经过充分研究的中欧动物群中，物种识别也可能非常困难。一个全面的、分类学上精心策划的DNA条形码文库可以弥补这一缺陷，也可以将数百年的传统知识与下一代测序技术联系起来。然而，这样一个甲虫库是失踪的日期。这项研究提供了全球最大的DNA条形码参考库鞘翅目15948个人属于3514个明确的物种（53%的德国动物群）与代表103个家庭（94%）中的97。这项研究是第一个全面的区域测试的效率DNA条形码的甲虫与重点在德国。从63%的分析标本（25294中的15948份）中回收了500 bp的序列，并从另外997份标本中回收了短序列。虽然大多数标本（92.2%）可以明确地分配到一个单一的已知物种的序列多样性在CO1，1089标本（6.8%）被分配到一个以上的条形码索引号（BIN），创建395个BIN需要进一步研究，以确定他们是否代表神秘的物种，线粒体基因渗入，或只是在广泛分布的物种的区域变异。我们发现409个标本（2.6%），共享一个BIN分配与另一个物种，大多数涉及一对密切相关的物种43个BIN参与。这些类群中的大多数被条形码分开，尽管序列差异很低。只有155个标本（0.97%）显示相同或重叠的集群。
Beetles are the most diverse group of animals and are crucial for ecosystem functioning. In many countries, they are well established for environmental impact assessment, but even in the well-studied Central European fauna, species identification can be very difficult. A comprehensive and taxonomically well-curated DNA barcode library could remedy this deficit and could also link hundreds of years of traditional knowledge with next generation sequencing technology. However, such a beetle library is missing to date. This study provides the globally largest DNA barcode reference library for Coleoptera for 15948 individuals belonging to 3514 well-identified species (53% of the German fauna) with representatives from 97 of 103 families (94%). This study is the first comprehensive regional test of the efficiency of DNA barcoding for beetles with a focus on Germany. Sequences 500 bp were recovered from 63% of the specimens analysed (15948 of 25294) with short sequences from another 997 specimens. Whereas most specimens (92.2%) could be unambiguously assigned to a single known species by sequence diversity at CO1, 1089 specimens (6.8%) were assigned to more than one Barcode Index Number (BIN), creating 395 BINs which need further study to ascertain if they represent cryptic species, mitochondrial introgression, or simply regional variation in widespread species. We found 409 specimens (2.6%) that shared a BIN assignment with another species, most involving a pair of closely allied species as 43 BINs were involved. Most of these taxa were separated by barcodes although sequence divergences were low. Only 155 specimens (0.97%) show identical or overlapping clusters.