MicroRNA-25 functions as a potential tumor suppressor in colon cancer by targeting Smad7
MicroRNA-25 functions as a potential tumor suppressor in colon cancer by targeting Smad7
复制标题
MicroRNA-25 通过靶向 Smad7 作为结肠癌的潜在肿瘤抑制剂
DOI：
10.1016/j.canlet.2013.02.029
复制
发表时间：
2013-07-10
期刊：
影响因子：
9.7
通讯作者：
Gao, Xu
中科院分区：
文献类型：
--
作者：
Li, Qiang;Zou, Chaoxia;Gao, Xu
由于它是与 25 簇相似的 miR-106b 的成员，因此 microRNA-25 (miR-25) 已知在人类癌症中失调。然而，miR-25在结肠癌中的表达和作用仍不清楚。在这项研究中，发现与匹配的非肿瘤性粘膜组织相比，miR-25 在人类结肠癌组织中下调。功能研究表明，恢复 miR-25 表达可抑制细胞增殖和迁移。相反，抑制miR-25可以促进细胞的增殖和迁移能力。 miR-25 的稳定过表达也抑制体内结肠癌细胞异种移植物的生长。此外，利用生物信息学预测和实验验证将 Smad7 确定为 miR-25 的直接靶标。功能反向实验表明，miR-25 的抗肿瘤作用可能是通过抑制 Smad7 介导的。这些结果表明，miR-25 可能通过靶向结肠癌中的 Smad7 发挥肿瘤抑制因子的作用。因此，miR-25可以作为癌症治疗的潜在治疗剂或靶点。皇冠版权所有 (c) 2013 由 Elsevier Ireland Ltd 出版。保留所有权利。
Because it is a member of the miR-106b similar to 25 cluster, microRNA-25 (miR-25) is known to be dysregulated in human cancers. However, the expression and role of miR-25 in colon cancer remain unclear. In this study, miR-25 was found to be down-regulated in human colon cancer tissues when compared to those in matched, non-neoplastic mucosa tissues. Functional studies revealed that restoration of miR-25 expression inhibited cell proliferation and migration. In contrast, miR-25 inhibition could promote the proliferation and migratory ability of cells. Stable over-expression of miR-25 also suppressed the growth of colon cancer-cell xenografts in vivo. Furthermore, bioinformatic predictions and experimental validation were used to identify Smad7 as a direct target of miR-25. Functional reverse experiments indicated that the antitumor effects of miR-25 were probably mediated by its repression of Smad7. These results suggest that miR-25 may function as a tumor suppressor by targeting Smad7 in colon cancer. Thus, miR-25 may serve as a potential therapeutic agent or target for cancer therapy. Crown Copyright (c) 2013 Published by Elsevier Ireland Ltd. All rights reserved.