Lung injury pathways: Adenosine receptor 2B signaling limits development of ischemic bronchiolitis obliterans organizing pneumonia.
Lung injury pathways: Adenosine receptor 2B signaling limits development of ischemic bronchiolitis obliterans organizing pneumonia.
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DOI：
10.1080/01902148.2017.1286697
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发表时间：
2017-03
影响因子：
1.7
通讯作者：
Jacobs ER
中科院分区：
文献类型：
--
作者：
Densmore JC;Schaid TR;Jeziorczak PM;Medhora M;Audi S;Nayak S;Auchampach J;Dwinell MR;Geurts AM;Jacobs ER
在单侧肺缺血引起的闭塞性细支气管炎机化性肺炎 (BOOP) 中研究了腺苷信号传导。通过左主肺动脉或完全肺门结扎实现缺血。研究人员对 Sprague Dawley (SD) 大鼠、Dahl 盐敏感 (SS) 大鼠和含有 A2B 腺苷受体基因 (Adora2b) 突变的 SS 突变大鼠品系进行了研究。通过 HPLC 测量支气管肺泡灌洗液 (BAL) 中的腺苷浓度。对 A2A (A2AAR) 和 A2B 腺苷受体 (A2BAR) mRNA 和蛋白质进行定量。单侧 PA 结扎后 24 小时，SD 大鼠缺血肺的 BAL 腺苷浓度相对于对侧肺有所增加。 PA 连接后，A2BAR mRNA 和蛋白质浓度增加，而缺血肺中的负调节 microRNA miR27a 减少。缺血后 A2AAR mRNA 和蛋白质浓度保持不变。 SS 大鼠 PA 结扎肺中 A2BAR 蛋白在 7 天后以及 SD 大鼠肺门完全结扎后 4 小时后增加。与 SS 大鼠相比，SS-Adora2b 突变体表现出更大程度的 BOOP 和更大的炎症变化。单侧肺缺血后 A2BAR 和腺苷增加以及 A2BAR 突变大鼠中 BOOP 增加，表明 A2BAR 信号传导在对抗缺血性肺损伤中具有保护作用。
Adenosine signaling was studied in bronchiolitis obliterans organizing pneumonia (BOOP) resulting from unilateral lung ischemia. Ischemia was achieved by either left main pulmonary artery or complete hilar ligation. Sprague Dawley (SD) rats, Dahl salt sensitive (SS) rats and SS mutant rat strain containing a mutation in the A2B adenosine receptor gene (Adora2b) were studied. Adenosine concentrations were measured in bronchoalveolar lavage (BAL) by HPLC. A2A (A2AAR) and A2B adenosine receptor (A2BAR) mRNA and protein were quantified. 24h after unilateral PA ligation, BAL adenosine concentrations from ischemic lungs were increased relative to contralateral lungs in SD rats. A2BAR mRNA and protein concentrations were increased after PA ligation while miR27a, a negatively regulating microRNA, was decreased in ischemic lungs. A2AAR mRNA and protein concentrations remained unchanged following ischemia. A2BAR protein was increased in PA ligated lungs of SS rats after 7d, and 4h after complete hilar ligation in SD rats. SS-Adora2b mutants showed a greater extent of BOOP relative to SS rats, and greater inflammatory changes. Increased A2BAR and adenosine following unilateral lung ischemia as well as more BOOP in A2BAR mutant rats implicate a protective role for A2BAR signaling in countering ischemic lung injury.
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DOI：
10.4049/jimmunol.1401957
发表时间：
2015-08-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hoegl S;Brodsky KS;Blackburn MR;Karmouty-Quintana H;Zwissler B;Eltzschig HK
通讯作者：
Eltzschig HK
DOI：
10.1016/j.jtcvs.2007.08.041
发表时间：
2008-01-01
影响因子：
6
作者：
Gazoni, Leo M.;Laubach, Victor E.;Kron, Irving L.
通讯作者：
Kron, Irving L.
影响因子：
5
作者：
Impellizzeri, Daniela;Di Paola, Rosanna;Cuzzocrea, Salvatore
通讯作者：
Cuzzocrea, Salvatore
影响因子：
4.8
作者：
Kong, Tianqing;Westerman, Karen A.;Colgan, Sean P.
通讯作者：
Colgan, Sean P.
DOI：
10.1126/science.1172447
发表时间：
2009-07-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Geurts AM;Cost GJ;Freyvert Y;Zeitler B;Miller JC;Choi VM;Jenkins SS;Wood A;Cui X;Meng X;Vincent A;Lam S;Michalkiewicz M;Schilling R;Foeckler J;Kalloway S;Weiler H;Ménoret S;Anegon I;Davis GD;Zhang L;Rebar EJ;Gregory PD;Urnov FD;Jacob HJ;Buelow R
通讯作者：
Buelow R