Specific microbes of saliva and vaginal fluid of Guangdong Han females based on 16S rDNA high-throughput sequencing
Specific microbes of saliva and vaginal fluid of Guangdong Han females based on 16S rDNA high-throughput sequencing
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基于16S rDNA高通量测序的广东汉族女性唾液和阴道液特异性微生物
DOI：
10.1007/s00414-018-1986-2
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发表时间：
2019-05-01
影响因子：
2.1
通讯作者：
Chen, Ling
中科院分区：
文献类型：
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作者：
Huang, Hongyan;Yao, Ting;Chen, Ling
阴道液和唾液在法医学中具有重要意义。阴道液或唾液的鉴定在刑事案件中尤为重要。微生物被认为是体液鉴定的一种有前途的标记物。本研究收集了18例广东汉族健康妇女的唾液和阴道液各18份。采用16S rDNA高通量测序法对上述样品进行微生物分析。结果表明，唾液中比例超过1%的微生物分布于12个属和57个操作分类单位（OTU），阴道液中比例超过1%的微生物分布于4个属和9个OTU。唾液中占主导地位的微生物与阴道液中占主导地位的微生物大不相同。采用线性判别分析（LDA）效应量（LEfSe）算法筛选出所研究样本的特定微生物，结果显示唾液样本中的特定微生物为副流感嗜血杆菌、小韦荣球菌和聚集杆菌，而阴道液中的特定微生物为惰性乳杆菌。
Vaginal fluid and saliva are of great importance in forensic sciences. The identification of vaginal fluid or saliva is especially important in criminal cases. Microbes are considered as a promising marker for the identification of body fluids. In this study, 18 salivary fluids and 18 vaginal fluid samples were collected from 18 healthy women of the Han population in Guangdong province, China. The microbes of the above samples were analyzed by 16S rDNA high-throughput sequencing. The results showed that the microbes whose proportions are over 1% in saliva samples distributed across 12 genera and 57 operational taxonomic units (OTUs), and in vaginal fluid distributed across 4 genera and 9 OTUs. The microbes that dominated in saliva were quite different from those dominated in vaginal fluids. The linear discriminant analysis (LDA) effect size (LEfSe) algorithm was used to screen out the specific microbes of the studied samples, and the results showed that the specific microbes in saliva samples are Haemophilus parainfluenzae, Veillonella parvula, and Aggregatibacter segnis, while in vaginal fluid is Lactobacillus iners.