RGD-Modified Nanofibers Enhance Outcomes in Rats after Sciatic Nerve Injury
RGD-Modified Nanofibers Enhance Outcomes in Rats after Sciatic Nerve Injury
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DOI：
10.3390/jfb10020024
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发表时间：
2019-06-01
影响因子：
4.8
通讯作者：
Becker, Matthew L.
中科院分区：
文献类型：
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作者：
Cavanaugh, McKay;Silantyeva, Elena;Becker, Matthew L.
需要手术的神经损伤是一个严重的问题，因为没有良好的临床替代自体移植。迫切需要组织工程策略来提供一种替代方案。在这项研究中，我们利用排列好的纳米纤维，用生物活性多肽RGD进行点击修饰，用于大鼠坐骨神经修复。用空导管或填充非功能化排列纳米纤维或RGD功能化排列纳米纤维的导管修复动物坐骨神经13 mm的缺损处，为期6周。组织学分析显示，排列整齐的纳米纤维促进了细胞的渗透和神经修复。RGD功能化的纳米纤维可以减少肌肉萎缩。在恢复的六周期间，这些动物接受了运动和感觉测试。RGD功能化纳米纤维组的感觉恢复在第4周有所改善，而其他组需要6周才能在受伤后表现出改善。因此，功能化纳米纤维的使用提供了有助于体内神经修复的线索，并应被视为未来的修复策略。
Nerve injuries requiring surgery are a significant problem without good clinical alternatives to the autograft. Tissue engineering strategies are critically needed to provide an alternative. In this study, we utilized aligned nanofibers that were click-modified with the bioactive peptide RGD for rat sciatic nerve repair. Empty conduits or conduits filled with either non-functionalized aligned nanofibers or RGD-functionalized aligned nanofibers were used to repair a 13 mm gap in the rat sciatic nerve of animals for six weeks. The aligned nanofibers encouraged cell infiltration and nerve repair as shown by histological analysis. RGD-functionalized nanofibers reduced muscle atrophy. During the six weeks of recovery, the animals were subjected to motor and sensory tests. Sensory recovery was improved in the RGD-functionalized nanofiber group by week 4, while other groups needed six weeks to show improvement after injury. Thus, the use of functionalized nanofibers provides cues that aid in in vivo nerve repair and should be considered as a future repair strategy.