Superoxide radical production in chicken skeletal muscle induced by acute heat stress
Superoxide radical production in chicken skeletal muscle induced by acute heat stress
复制标题
DOI：
10.1093/ps/84.2.307
复制
发表时间：
2005-02-01
期刊：
影响因子：
4.4
通讯作者：
Toyomizu, M
中科院分区：
文献类型：
--
作者：
Mujahid, A;Yoshiki, Y;Toyomizu, M
热应激是家禽的主要关注点，特别是在世界上的炎热地区，因为热应激导致生长性能差、免疫抑制和高死亡率。采用电子自旋共振（ESR）技术和光泽精化学发光（LDCL）技术，研究了急性热应激（n = 4 ~ 8）鸡骨骼肌线粒体超氧阴离子（O21）的产生。对照组和急性热处理肉鸡线粒体悬浮液的ESR谱显示出与次黄嘌呤氧化酶体系中DMPO-O-2（-）加合物相似的超精细偶联常数（aN = 1.44 mT，aH β = 0.12 mT，aH β = 0.11，mT）。热暴露导致DMPO-O-2（.-）信号了结果表明，LDCL显著增强了热应激处理的肉鸡骨骼肌线粒体中超氧化物的产生，而在产蛋鸡中没有观察到这种增加。在前一种情况下，超氧化物产生的增强与直肠和肌肉温度的热诱导增量有关，导致显着的体重减轻。相比之下，后一种情况显示温度没有增加，但体重增加略有下降。超氧化物产生的百分比增加的存在下，羧基乙酸，腺嘌呤核苷酸转运蛋白（ANT）的特异性抑制剂，是相同的骨骼肌线粒体从肉和产蛋型鸡从控制或热应激处理组。这一发现表明ANT在热应激条件下调节活性氧通量无关。本研究首次提供了肉鸡骨骼肌线粒体在急性热应激反应中产生超氧阴离子的证据。
Heat stress is of major concern for poultry, especially in the hot regions of the world because of the resulting poor growth performance, immunosuppression, and high mortality. To assess superoxide (021 production in mitochondria isolated from skeletal muscle of chickens (n = 4 to 8) exposed to acute heat stress, electron spin resonance (ESR) spectroscopy using 5,5-dimethyl-1-pyrroline N-oxide (DMPO) as a spin trap agent and lucigenin-derived chemiluminescence (LDCL) method were applied. ESR spectra of suspensions containing mitochondria from control and acute heat-treated meat-type chickens showed similar hyperfine coupling constants (aN = 1.44 mT, aHbeta = 0.12 mT, and aHbeta = 0.11, mT) to those of DMPO-O-2(-) adducts observed in a hypoxanthinexanthine oxidase system. Heat exposure resulted in enhancement of the DMPO-O-2(.-) signal. The results using LDCL showed significantly enhanced superoxide production in heat stress-treated skeletal muscle mitochondria of meat-type chickens, whereas no such increase was observed in laying chickens. The enhancement of superoxide production in the former case was associated with heat-induced increments in rectal and muscle temperatures, leading to significant body weight loss. In contrast, the latter case showed no increase in temperatures, although there was a slight decrease in body weight gain. Percentage increases of superoxide production in the presence of carboxyatractylate, a specific inhibitor of adenine nucleotide translocator (ANT), were the same for skeletal muscle mitochondria from meat- and laying-type chickens from the control or heat stress-treated group. This finding suggests the irrelevance of ANT in the regulation of reactive oxygen species flux under heat stress conditions. The study provides the first evidence Of Superoxide anion production in the skeletal muscle mitochondria of meat-type chickens in response to acute heat stress.