Neuropeptide Y in trigeminal ganglion following chronic constriction injury of the rat infraorbital nerve: is there correlation to somatosensory parameters?
Neuropeptide Y in trigeminal ganglion following chronic constriction injury of the rat infraorbital nerve: is there correlation to somatosensory parameters?
复制标题
DOI：
10.1016/s0304-3959(00)00417-6
复制
发表时间：
2001-03-01
期刊：
影响因子：
7.4
通讯作者：
Iadarola, MJ
中科院分区：
文献类型：
--
作者：
Benoliel, R;Eliav, E;Iadarola, MJ
本研究的目的是探讨眶下神经损伤后三叉神经节中神经肽Y（NPY）的水平。实验分为3组：单侧眶下神经慢性压迫性损伤（CCI）组（n = 13）、单纯眶下神经压迫组（n = 13）和未手术对照组（n = 8）。眶下神经CCI在9-12天内引起明显的痛觉过敏和异常性疼痛。在第6天，对7只大鼠实施安乐死，并收获三叉神经节用于免疫细胞化学（ICC）研究。研究在第14天结束，此时所有大鼠均被安乐死，并收获其神经节用于ICC和放射免疫测定（RIA）研究。在6天时，当大鼠没有表现出疼痛相关行为时，在操纵和CCI大鼠的同侧神经节中观察到NPY水平的增加。在第14天，ICC和RIA均检测到CCI和操作大鼠同侧神经节中的NPY水平显著增加，但在未操作的对照组中没有。根据这些发现，讨论了NPY在疼痛调制和神经损伤中的可能作用，（C）2001年国际疼痛研究协会。由Elsevier Science B. V.出版，版权所有。
The aim of this study was to investigate neuropeptide Y (NPY) levels in trigeminal ganglia following infraorbital nerve injury. Two experimental procedures were performed in three groups of rats: a unilateral chronic constriction injury (CCI) to the infraorbital nerve (n = 13), nerve manipulation without CCI (n = 13) and unoperated controls (n = 8), All rats underwent baseline and regular assessment of mechanical withdrawal threshold (Von Frey) and reaction to pin prick as well as free behavior evaluations. CCI to the infraorbital nerve induced significant hyperalgesia and allodynia within 9-12 days. At 6 days seven rats were euthanized and trigeminal ganglia harvested for immunocytochemical (ICC) studies. The study was ended at 14 days when all rats were euthanized and their ganglia harvested for ICC and radioimmunoassay (RIA) studies. An increase in NPY levels was seen in the ipsilateral ganglia of manipulated and CCI rats at 6 days, when rats displayed no pain-related behavior. At 14 days, ICC and RIA both detected significant increases in NPY levels in the ipsilateral ganglia of CCI and manipulated rats but not in unoperated controls. The possible roles of NPY in pain modulation and nerve injury are discussed in light of these findings, (C) 2001 International Association for the Study of Pain. Published by Elsevier Science B.V. All rights reserved.