Fiber types in normal and neonatally denervated fast muscles of the rat: immunocytochemical study with an antimyosin monoclonal antibody.
Fiber types in normal and neonatally denervated fast muscles of the rat: immunocytochemical study with an antimyosin monoclonal antibody.
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正常和新生儿去神经大鼠快肌中的纤维类型：使用抗肌球蛋白单克隆抗体进行免疫细胞化学研究。
DOI：
10.1016/0014-4886(87)90104-x
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发表时间：
1987
影响因子：
5.3
通讯作者：
Shafiq,SA
中科院分区：
文献类型：
--
作者：
Leung,B;Kula,RW;Shafiq,SA
用肌球蛋白ATP酶组织化学和免疫细胞化学方法，用单克隆抗体HM-1.2，比较了正常和去神经支配的大鼠腓肠肌肌纤维类型的分化。HM-1.2对快速肌球蛋白重链的特异性通过放射免疫分析、免疫放射自显影和间接免疫荧光技术测定。正常1月龄和成年大鼠腓肠肌IIB型（快速糖酵解）纤维可明显分为3个亚型，肌球蛋白重链特异性单克隆抗体免疫荧光染色可将其分级。在新生大鼠腓肠肌中，所有纤维与单克隆抗体均呈阴性。从阴性到三级免疫反应性的过渡发生在出生后1至2周。然而，新生儿腓肠肌去神经支配后，观察到肌球蛋白重链的单克隆抗体免疫荧光染色呈均匀阳性，无亚型分化。因此，这项研究，给出了明确的免疫细胞化学证据，IIB型肌纤维是异质性相对于他们的肌球蛋白亚型，这种异质性的表达是依赖于正常发育的影响，运动神经支配的肌纤维。
The differentiation of fiber types in normal and neonatally denervated gastrocnemius muscles of the rat was compared by myosin ATPase histochemistry and immunocytochemistry using a monoclonal antibody, HM-1.2. The specificity of HM-1.2 for the fast myosin heavy chain was determined by radioimmunoassay, immunoautoradiography, and indirect immunofluorescence techniques. In normal 1-month-old and adult rats, the type IIB (fast glycolytic) fibers of the gastrocnemius could be clearly divided into three subtypes by their graded immunofluorescence staining with the myosin heavy chain-specific monoclonal antibody. In the gastrocnemius muscle of the newborn rat, all fibers were negative with the monoclonal antibody. The transition from negative to three grades of immunoreactivity occurred 1 to 2 weeks postnatally. After neonatal denervation of the gastrocnemius muscle, however, uniformly positive monoclonal antibody immunofluorescence staining for the myosin heavy chain was observed without subtype differentiation. This study, thus, gave clear immunocytochemical evidence that the type IIB muscle fibers are heterogeneous with respect to their myosin isoform and that the expression of this heterogeneity is dependent on the normal developmental influence of motor innervation on the muscle fibers.