Georgenia sediminis sp nov., a moderately thermophilic actinobacterium isolated from sediment
Georgenia sediminis sp nov., a moderately thermophilic actinobacterium isolated from sediment
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乔治亚·塞迪米尼斯 sp.
DOI：
10.1099/ijs.0.051714-0
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发表时间：
2013-11-01
影响因子：
2.8
通讯作者：
Zhang, Si
中科院分区：
文献类型：
--
作者：
You, Zhi-Qing;Li, Jie;Zhang, Si
从南海沉积物中分离到一株革兰氏染色阳性放线菌，命名为SCSIO 15020（T），并对其进行了多相鉴定。生长的温度范围是24-60 ℃，最佳生长发生在50 ℃。生长的pH范围为6-10（最适pH 8-9）。生长的NaCl浓度范围为0 5%（w/v）。肽聚糖类型为A4 α。极性脂质含有二磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和未知极性脂质。主要的甲萘醌是MK-8（H-4）; MK-7（H-4）作为次要成分存在。SCSIO 15020 T菌株的DNA G+C含量为73.2 mol%，主要脂肪酸为anteiso-C-15：0、iso-C-15：0和iso-C-16：0。基于16 S rRNA基因序列的系统发育分析表明，菌株SCSIO 15020（T）属于Georgenia属，与Georgenia muralis 1A-C-T（96.3%）、Georgenia thermotolerans TT 02 -04（T）（95.7%）和Georgenia ruanii YIM 004（T）（95.6%）的亲缘关系最近。根据目前多相研究的证据，菌株SCSIO 15020（T）被认为是Georgenia属的一个新种，并建议将其命名为Georgenia sediminis sp. nov.。模式菌株为SCSIO 15020（T）（=DSM 25884（T）= NBRC 108941（T））。
A Gram-stain-positive actinobacterium, designated strain SCSIO 15020(T), was isolated from sediment of the South China Sea, and characterized by using a polyphasic approach. The temperature range for growth was 24-60 degrees C, with optimal growth occurring at 50 C. The pH range for growth was 6-10 (optimum pH 8-9). The NaCl concentration range for growth was 0 5 % (w/v). The peptidoglycan type was A4 alpha. Polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unknown polar lipid. The major menaquinone was MK-8(H-4); MK-7(H-4) was present as a minor component. The major fatty acids (>5 %) were anteiso-C-15:0, iso-C-15:0 and iso-C-16:0 The DNA G+C content of strain SCSIO 15020T was 73.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCSIO 15020(T) belonged to the genus Georgenia, with the closest neighbours being Georgenia muralis 1A-C-T (96.3% similarity), Georgenia thermotolerans TT02-04(T) (95.7%) and Georgenia ruanii YIM 004(T) (95.6%). Based on evidence from the present polyphasic study, strain SCSIO 15020(T) is considered to represent a novel species of the genus Georgenia, for which the name Georgenia sediminis sp. nov. is proposed. The type strain is SCSIO 15020(T) (=DSM 25884(T)= NBRC 108941(T)).