Intestinal adaptation and enterocyte apoptosis following small bowel resection is p53 independent.
Intestinal adaptation and enterocyte apoptosis following small bowel resection is p53 independent.
复制标题
小肠切除后的肠适应和肠上皮细胞凋亡与 p53 无关。
DOI：
10.1152/ajpgi.1999.277.3.g717
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Warner,BW
中科院分区：
文献类型：
--
作者：
Shin,CE;FalconeJr,RA;Kemp,CJ;Erwin,CR;Litvak,DA;Evers,BM;Warner,BW
小肠切除术（SBR）后的适应性变化是肠上皮细胞增殖和凋亡的信号。由于p53诱导的p21 waf 1/cip 1可能对许多细胞的凋亡很重要，我们假设这些基因是适应过程中肠上皮细胞凋亡增加所必需的。雄性C57 BL/6（野生型）或p53缺失小鼠进行50%近端SBR或假手术（肠横断-再吻合术）。5天后在回肠中测量适应（DNA-蛋白质含量、绒毛高度-隐窝深度、肠上皮细胞增殖）、凋亡小体的出现以及p53和p21 waf 1/cip 1蛋白表达。适应是相当的SBR后，在野生型和p53基因敲除小鼠监测回肠DNA蛋白含量，绒毛高度，肠上皮细胞增殖显着增加。在野生型和p53基因敲除小鼠中，SBR后隐窝凋亡小体的数量均显著增加。在p53基因敲除小鼠中，SBR显著诱导p21 waf 1/cip 1蛋白在绒毛肠上皮细胞中的表达。p21 waf 1/cip 1的p53非依赖性诱导可能解释了野生型和p53缺失小鼠对SBR的相似肠道反应。肠切除术后肠适应和肠细胞凋亡增加通过p53非依赖性机制发生。
Adaptation following small bowel resection (SBR) signals enterocyte proliferation and apoptosis. Because p53-induced p21waf1/cip1may be important for apoptosis in many cells, we hypothesized that these genes are required for increased enterocyte apoptosis during adaptation. Male C57BL/6 (wild-type) or p53-null mice underwent 50% proximal SBR or sham operation (bowel transection-reanastomosis). Adaptation (DNA-protein content, villus height-crypt depth, enterocyte proliferation), appearance of apoptotic bodies, and p53 and p21waf1/cip1protein expression were measured in the ileum after 5 days. Adaptation was equivalent after SBR in both wild-type and p53-null mice as monitored by significantly increased ileal DNA-protein content, villus height, and enterocyte proliferation. The number of crypt apoptotic bodies increased significantly after SBR evenly in both wild-type and p53-null mice. In the p53-null mice, SBR substantially induced the expression of p21waf1/cip1protein in villus enterocytes. The p53-independent induction of p21waf1/cip1may account for the similar intestinal response to SBR between wild-type and p53-null mice. Intestinal adaptation and increased enterocyte apoptosis following intestinal resection occur via a p53-independent mechanism.
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在三种人胃癌细胞系中，高温诱导的细胞凋亡以 p53 基因依赖性和非依赖性方式发生。
DOI：
--
发表时间：
1999
期刊：
Oncology Report
影响因子：
--
作者：
A. Goto;K. Shomori;T. Ohkumo;F. Tanaka;Kenzo Sato;Hisao Ito
通讯作者：
Hisao Ito
影响因子：
2.2
作者：
Helmrath, MA;Shin, CE;Warner, BW
通讯作者：
Warner, BW
肠道适应（两部分中的第二部分）。
影响因子：
158.5
作者：
Williamson Rc
通讯作者：
Williamson Rc
影响因子：
3.8
作者：
Ehrenfried, JA;Ko, TC;Evers, BM
通讯作者：
Evers, BM
影响因子：
8
作者：
Esteve, P;Embade, N;Lacal, JC
通讯作者：
Lacal, JC