Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure
Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure
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DOI：
10.1128/aem.01039-07
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发表时间：
2008-01-01
影响因子：
4.4
通讯作者：
Uyttendaele, Mieke
中科院分区：
文献类型：
--
作者：
Baert, Leen;Wobus, Christiane E.;Uyttendaele, Mieke
采用实时荧光定量逆转录-聚合酶链式反应(RT-PCR)检测小鼠诺如病毒1RNA，并与80℃高温暴露前后空斑试验的感染性进行相关性分析。在目前的研究中没有发现相关性。此外，热灭活对病毒感染性的有害影响要比对病毒基因组的完整性影响大得多。
The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80 degrees C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.