Advanced optical tweezers for the study of cellular and molecular biomechanics
Advanced optical tweezers for the study of cellular and molecular biomechanics
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DOI：
10.1109/tbme.2002.805463
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发表时间：
2003-01-01
影响因子：
4.6
通讯作者：
Hunt, AJ
中科院分区：
文献类型：
--
作者：
Brouhard, GJ;Schek, HT;Hunt, AJ
光镊是研究细胞和分子生物力学的重要工具。我们推出了一款坚固的光镊设备，具有先进的功能，包括：多个光陷阱、声光陷阱转向和后焦平面干涉测量位置检测。我们将这些功能集成到正置显微镜中，同时不影响其功能（微分干涉显微镜、荧光显微镜等）。声光偏转器 (AOD) 控制每个光束，并可以创建多个分时陷阱。介绍了位置检测、力校准和 AOD 性能。该系统可以检测亚纳米位移和低于 0.1 pN 的力。
Optical tweezers are an important tool for studying cellular and molecular biomechanics. We present a robust optical tweezers device with advanced features including: multiple optical traps, acousto-optic trap steering, and back focal plane interferometry position detection. We integrate these features into an upright microscope, with no compromise to its capabilities (differential interference contrast microscopy, fluorescence microscopy, etc.). Acousto-optic deflectors (AODs) steer each beam and can create multiple time-shared traps. Position detection, force calibrations and AOD performance are presented. The system can detect subnanometer displacements and forces below 0.1 pN.