Lycopene attenuates oxidative stress induced experimental cataract development: An in vitro and in vivo study
Lycopene attenuates oxidative stress induced experimental cataract development: An in vitro and in vivo study
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DOI：
10.1016/s0899-9007(03)00140-0
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发表时间：
2003-09-01
期刊：
影响因子：
4.4
通讯作者：
Verma, SD
中科院分区：
文献类型：
--
作者：
Gupta, SK;Trivedi, D;Verma, SD
目的：番茄红素是一种营养性抗氧化剂，评价其抗白内障的潜力，以进一步确定其在白内障预防中的作用。番茄红素调节生化参数的能力进行了研究，在体外研究。去核大鼠晶状体保持在含有Dulbecco改良Eagles培养基的器官培养物中，单独或另外含有100 μ M亚硒酸盐，并分别作为正常组和对照组。对于试验组，对照培养基补充有10 μ M lycopetie。将晶状体在37 ℃下孵育24 h。在孵育期结束时，检查晶状体的形态变化，并估计生化参数，如还原型谷胱甘肽、脂质过氧化产物丙二醛和抗氧化酶谷胱甘肽过氧化物酶、谷胱甘肽S-转移酶、超氧化物歧化酶和过氧化氢酶。用亚硒酸钠（25 mumoles/kg体重）皮下注射9日龄大鼠诱发体内亚硒酸盐性白内障。在亚硒酸盐攻击之前，试验组的大鼠被注射番茄红素（200 μ g/kg体重，腹膜内）4 μ g/kg。观察大鼠首次睁眼时白内障的发生率。以30%半乳糖喂养大鼠，诱发半乳糖性白内障。试验组的大鼠每天口服200 μ g/kg番茄红素，对照组的大鼠仅接受载体。白内障阶段定期进行分级。结果：谷胱甘肽水平下降（25%），丙二醛含量上升（32%），观察到在控制，而不是正常的镜头。在培养液中添加番茄红素显著（P < 0.001）恢复了谷胱甘肽和丙二醛的水平，在对照晶状体中也观察到抗氧化酶活性的显著降低。补充赖氨酸组的超氧化物歧化酶（P < 0.05）、过氧化氢酶（P < 0.01）和谷胱甘肽S-转移酶（P < 0.01）的活性显著恢复，而谷胱甘肽过氧化物酶（GSH过氧化物酶）活性无明显变化。番茄红素还能降低亚硒酸盐性白内障的发生率。试验组中只有9%的眼睛出现致密核混浊，而对照组中有83%。口服番茄红素可明显延缓半乳糖性白内障的发生和发展。只有35%的眼睛发展成熟的白内障，而不是100%的对照group.CONCLUSIONS：番茄红素保护实验性白内障的发展凭借其抗氧化性能，它可能是有用的预防或治疗白内障。(C)Elsevier Inc. 2003.
OBJECTIVE: Lycopene, a nutritional antioxidant, was evaluated for its anticataract potential to further establish its role in cataract prevention.METHODS. The ability of lycopene to modulate the biochemical parameters was investigated by in vitro studies. Enucleated rat lenses were maintained in organ culture containing Dulbecco's Modified Eagles Medium alone or in addition with 100 muM selenite and served as the normal and control groups, respectively. For the test group, the control medium was supplemented with 10 muM lycopetie. The lenses were incubated for 24 h at 37degreesC At the end of the incubation period, the lenses were examined for morphologic variation, and biochemical parameters such as reduced glutathione, the lipid peroxidation product malondialdehyde, and the antioxidant enzymes glutathione peroxidase, glutathione S-transferase, superoxide dismutase, and catalase were estimated. In vivo selenite cataract was induced in 9-d-old rats by subcutaneous injection of sodium selenite (25 mumoles/kg of body weight). The rats in the test group were injected with lycopene (200 mug/kg body weight, intraperitoneally) 4 It before the selenite challenge. The incidence of cataract was observed when the rats first opened their eyes. Galactose cataract was induced in rats by feeding 30% galactose in the diet. Rats in the test group were fed orally with 200 mug/kg of lycopene daily, and rats in the control group received only vehicle. Cataract stages were graded at regular intervals.RESULTS: A fall (25%) in the glutathione level and a rise (32%) in the malondialdehyde content were observed in control as opposed to normal lenses. Lycopene supplementation in the medium significantly (P < 0.001) restored glutathione and maloadialdehyde levels, A significant decrease in the activity of antioxidant enzymes also was observed in the control lenses. A significant restoration in the activities of superoxide dismutase (P < 0.05) and catalase and glutathione S-transferase (P < 0.01), with no effect on glutathione peroxidase, was observed in the lycopene-supplemented group. Lycopene also reduced the incidence of selenite cataract. Only 9% of the eyes in the test group developed dense nuclear opacity as opposed to 83% in the control group. A significant delay in the onset and progression of galactose cataract was observed with oral feeding of lycopene. Only 35% of the eyes developed mature cataract as opposed to 100% in the control group.CONCLUSIONS: Lycopene protects against experimental cataract development by virtue of its antioxidant properties, and it may be useful for prophylaxis or therapy against cataracts. (C) Elsevier Inc. 2003.