Presence of two novel cardiolipins in the halophilic archaeal community in the crystallizer brines from the salterns of Margherita di Savoia (Italy) and Eilat (Israel)
Presence of two novel cardiolipins in the halophilic archaeal community in the crystallizer brines from the salterns of Margherita di Savoia (Italy) and Eilat (Israel)
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DOI：
10.1007/s00792-002-0279-2
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发表时间：
2002-12-01
期刊：
影响因子：
2.9
通讯作者：
Oren, A
中科院分区：
文献类型：
--
作者：
Lattanzio, VMT;Corcelli, A;Oren, A
最近在盐杆菌中检测到两种新型心磷脂衍生物，即双磷脂酰甘油 (BPG) 的古菌类似物和糖心磷脂 (GlyC)。发现 GlyC 与细菌视紫红质紧密结合。为了获得有关这些古细菌心磷脂的存在和分布的信息，我们通过电喷雾电离质谱分析了从玛格丽塔迪萨沃亚（意大利）和埃拉特（以色列）盐场的结晶池中提取的脂质以及从盐杆菌科代表性物种的培养物中提取的脂质。 BPG 作为少量脂质成分存在于从 Margherita di Savoia 和埃拉特盐场的生物质中提取的脂质中，而 GlyC 仅在 Margherita di Savoia 的生物质提取物中检测到。这两种化合物在通过蒸馏水透析细胞获得的膜部分中富集。我们在所有测试的盐杆菌科成员中都检测到了 BPG，但迄今为止仅在盐杆菌属和盐弧菌属中发现了 GlyC。硫酸化二糖基二醚是在两个盐场的生物质中检测到的主要糖脂。
Two novel cardiolipin derivatives were recently detected in Halobacterium salinarum, namely an archaeal analog of bisphosphatidylglycerol (BPG) and a glyco-cardiolipin (GlyC). GlyC was found to be tightly bound to bacteriorhodopsin. To obtain information on the presence and distribution of these archaeal cardiolipins, we have analyzed the lipids extracted from the crystallizer ponds of the salterns of Margherita di Savoia (Italy) and Eilat (Israel) and from cultures of representative species of the Halobacteriaceae by electrospray ionization mass spectrometry. BPG was present as a minor lipid component in the lipids extracted from the biomass of the Margherita di Savoia and the Eilat salterns, while GlyC was detected only in the extract of the biomass of Margherita di Savoia. Both compounds were enriched in the membrane fraction obtained by dialysis of the cells against distilled water. We detected BPG in all members of the Halobacteriaceae tested, but GlyC has so far been found only in the genera Halobacterium and Haloarcula. A sulfated diglycosyl diether was the major glycolipid detected in the biomass of both salterns.