Dietary protein modulates intrarenal distribution of renin and its mRNA during development.
Dietary protein modulates intrarenal distribution of renin and its mRNA during development.
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膳食蛋白质在发育过程中调节肾素及其 mRNA 的肾内分布。
DOI：
10.1152/ajprenal.1992.263.3.f427
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Gomez,RA
中科院分区：
文献类型：
--
作者：
Tufro-McReddie,A;Arrizurieta,EE;Brocca,S;Gomez,RA
为了确定整个发育过程中的高蛋白喂养是否会影响成年动物的肾脏生长、肾血流动力学以及肾素及其 mRNA 的肾内分布，雄性 Wistar 大鼠从断奶到 6 周或 12 周龄的研究期间，喂食含有 20% 蛋白质 [正常 (NP)，n = 12] 或 40% 蛋白质 [高 (HP)，n = 12] 的饮食。 6周龄和12周龄时，HP喂养的大鼠的肾脏重量、肾脏重量与体重的比率、皮质DNA含量和皮质蛋白质与DNA的比率高于NP喂养的大鼠。血管紧张素 II 1 型受体拮抗剂 Dup 753 减弱了体细胞和肾脏生长对 HP 的反应。在 6 周和 12 周龄时，HP 喂养的大鼠的肾小球滤过率和肾血浆流量高于 NP 喂养的大鼠。分别通过免疫细胞化学和原位杂交评估，两组之间肾素和肾素 mRNA 的肾内分布存在显着差异。在 NP 喂养的大鼠中，肾素和肾素 mRNA 局限于肾小球旁位置。在 HP 喂养的大鼠中，肾素及其 mRNA 沿着传入小动脉向近端延伸。 HP 喂养的大鼠中含有肾素或肾素 mRNA 的可见传入小动脉长度的百分比（分别为 60 +/- 3.2 和 61 +/- 3.9%）高于 NP 喂养的大鼠（39 +/- 2.5 和 33 +/- 0.6%；P 小于 0.05）。此外，HP 喂养的大鼠中含有肾素或肾素 mRNA 的近肾小球装置 (JGA) 的百分比（分别为 80 +/- 1.6 和 72 +/- 2%）高于 NP 喂养的大鼠（46 +/- 2.2 和 40 +/- 4%；P 小于 0.05）。（摘要截断为 250 字）
To determine whether high protein feeding throughout development affects renal growth, renal hemodynamics, and the intrarenal distribution of renin and its mRNA in the adult animal, male Wistar rats were fed diets containing either 20% protein [normal (NP), n = 12] or 40% protein [high (HP), n = 12] from weaning until studied at 6 or 12 wk of age. Kidney weight, kidney weight-to-body weight ratio, cortical DNA content, and cortical protein-to-DNA ratio were higher in HP- than in NP-fed rats at 6 and 12 wk of age. Somatic and kidney growth response to HP was blunted by angiotensin II type 1 receptor antagonist Dup 753. Glomerular filtration rate and renal plasma flow were higher in HP- than in NP-fed rats at 6 and 12 wk of age. The intrarenal distribution of renin and renin mRNA, assessed by immunocytochemistry and in situ hybridization, respectively, were markedly different between the two groups. In NP-fed rats, renin and renin mRNA were confined to a juxtaglomerular location. In HP-fed rats, renin and its mRNA extended proximally along the afferent arterioles. The percentage of visible afferent arteriolar length containing renin or renin mRNA was higher in HP-fed rats (60 +/- 3.2 and 61 +/- 3.9%, respectively) than in NP-fed rats (39 +/- 2.5 and 33 +/- 0.6%; P less than 0.05). Also, the percentage of juxtaglomerular apparatuses (JGAs) containing renin or renin mRNA was higher in HP-fed rats (80 +/- 1.6 and 72 +/- 2%, respectively), than in NP-fed rats (46 +/- 2.2 and 40 +/- 4%; P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)