Variants-250G/A and-514C/T in the LIPC gene are associated with hypertensive disorders of pregnancy in Chinese women
Variants-250G/A and-514C/T in the LIPC gene are associated with hypertensive disorders of pregnancy in Chinese women
复制标题
LIPC基因中的-250G/A和-514C/T变异与中国女性妊娠期高血压疾病相关。
DOI：
10.4238/2014.august.7.28
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发表时间：
2014-01-01
影响因子：
0.4
通讯作者：
Liu, H. K.
中科院分区：
文献类型：
--
作者：
Lin, H.;Yin, Z.;Liu, H. K.
我们检测了人肝脂酶(LIPC)基因启动子-250G/A(Rs2070895)和-514C/T(Rs1800588)多态性对中国人群血脂异常和妊娠期高血压疾病(HDCP)的影响。临床确诊的HDCP患者(N=321例)和健康孕妇(N=331例)采用聚合酶链式反应-限制性片段长度多态方法对两个LIPC单核苷酸多态(SNPs)进行基因分型。结果显示HDCP与甘油三酯、载脂蛋白A1、高密度脂蛋白胆固醇呈显著正相关(P<；0.05)，证实HDCP伴发血脂异常。在妊娠期高血压疾病患者中，总胆固醇和收缩压水平均与这两个SNPs相关(P≤0.004)，而HDCP和LIPC基因或等位基因之间无显著关联。两个SNPs在慢性阻塞性肺病患者(R(2)=0.867)和对照组(R(2)=0.91)中均存在显著连锁不平衡。轻度子痫前期(MPE)患者的体重指数(BMI)与-250g/A相关(P=0.01)。在MPE组中，突变纯合子-250AA基因携带者的BMI较高。总之，LIPC-250G/A和-514C/T变异影响GH患者的TC和SBP水平以及MPE组的BMI水平，尽管没有证据表明HDCP与LIPC等位基因、基因型或单倍型频率之间存在关联。
We examined the influence of the promoter polymorphisms -250G/A (rs2070895) and -514C/T (rs1800588) in the human hepatic lipase (LIPC) gene on dyslipidemia and hypertensive disorders complicating pregnancy (HDCP) in a Chinese population. Clinically defined HDCP patients (N = 321) and healthy pregnant women (N = 331) were recruited and genotyped using polymerase chain reaction-restriction fragment length polymorphism for the two LIPC single nucleotide polymorphisms (SNPs). The results showed significant relationships between HDCP and triglycerides, apolipoprotein A1, and high-density lipoprotein cholesterol (P < 0.05), which confirmed that HDCP was accompanied by dyslipidemia. The results also demonstrated that in gestational hypertension (GH) patients, both total cholesterol (TC) and systolic blood pressure (SBP) levels were related to the two SNPs (P ≤ 0.004), although no significant association was found between HDCP and LIPC genotypes or alleles. Significant linkage disequilibrium of the two SNPs was found in both HDCP patients (R(2) = 0.867) and controls (R(2) = 0.91). Body mass index (BMI) was associated with -250G/A in women with mild preeclampsia (MPE) (P = 0.01). Carriers of the mutant homozygote -250AA genotype presented higher BMI in the MPE group. In conclusion, the LIPC -250G/A and -514C/T variants influenced TC and SBP levels in GH patients and the BMI level in the MPE group, although there was no evidence to validate an association between HDCP and LIPC allele, genotype, or haplotype frequencies.