Decipher the molecular evolution and expression patterns of Cupin family genes in oilseed rape
Decipher the molecular evolution and expression patterns of Cupin family genes in oilseed rape
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破译油菜Cupin家族基因的分子进化和表达模式
DOI：
10.1016/j.ijbiomac.2022.12.150
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发表时间：
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期刊：
影响因子：
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通讯作者：
Cao J
中科院分区：
文献类型：
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作者：
Hu F;Ye Z;Zhang W;Fang D;Cao J
Cupin 蛋白参与植物生长和发育以及对各种胁迫的反应。在此，在Brassicanapus中总共鉴定了173个Cupinggenes，并分析了它们的分子进化和表达模式。这些基因被分为十组。基序和外显子-内含子结构表明在进化过程中各类群内具有高度保守性。BnaCupins分布在19条染色体上，其扩增主要由全基因组复制（WGD）和片段复制事件促成。BnaCupins在漫长的进化过程中经历了严格的纯化选择。同时，确定了一些正选择位点。表达模式和顺式元件分析表明BnaCupins在植物生长和逆境响应中发挥重要作用。此外，当用不同条件（寒冷、盐、干旱、IAA、ABA和6-BA）处理时，一些BnCupins的表达水平显着改变。通过蛋白质-蛋白质相互作用网络鉴定了一些 BnaCupin 相互作用蛋白，例如糖基水解酶 5 (GHs5)、碳水化合物激酶 (CHK)、ATP 依赖性 6-磷酸果糖激酶 (ATP-PFK)、S-腺苷甲硫氨酸合酶 (S-MAT) 和醛缩酶 II (ALD II)。它将有助于丰富我们对 B 中 Cupingene 家族的了解。为进一步研究其功能提供了基础。
Cupin proteins are involved in plant growth and development as well as in response to various stresses. Here, a total of 173Cupingenes were identified inBrassicanapus, and their molecular evolution and expression patterns were analyzed. These genes were classified into ten groups. Motif and exon-intron structure indicated a high degree of conservation within each group during evolution.BnaCupinswere distributed on 19 chromosomes and their expansion is mainly contributed by whole-genome duplication (WGD) and segmental duplication events.BnaCupinshave undergone severe purifying selection during a long evolutionary process. Meanwhile, some positive selection sites were identified. Expression patterns andcis-element analysis indicated thatBnaCupinsplay significant roles in plant growth and stress responses. In addition, the expression levels of someBnCupins were significantly altered when treated with different conditions (cold, salt, drought, IAA, ABA, and 6-BA). Some BnaCupin interacting proteins, such as glycosyl hydrolase5 (GHs5), carbohydrate kinase (CHKs), ATP-dependent 6-phosphofructokinase (ATP-PFK), S-adenosylmethionine synthase (S-MAT), and aldolase class II (ALD II), were identified by the protein-protein interaction network. It will contribute to enriching our knowledge of theCupingene family inB. napusand provide a basis for further studies of their functions.