Cell union and protein synthesis in conjugation of Blepharisma.
Cell union and protein synthesis in conjugation of Blepharisma.
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Blepharisma 结合中的细胞结合和蛋白质合成。
DOI：
10.1016/0014-4827(76)90323-2
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发表时间：
1976
影响因子：
3.7
通讯作者：
H. Honda
中科院分区：
文献类型：
--
作者：
A. Miyake;H. Honda
本文研究了中间型睑肿接合过程中蛋白质合成在细胞结合中的作用。为了避免由于接合中其他过程的发生而可能的并发症，使用了同型细胞结合，其中接合在细胞结合阶段停止而没有进一步的变化。用一种交配型的配子体处理另一种交配型的细胞约2小时可诱导这种结合。细胞结合的诱导有规律地伴随着蛋白质合成的增加，其在gamone处理开始后5分钟开始并持续约2小时。当蛋白质合成被放线菌酮抑制时，细胞结合也被抑制。两种抑制的程度密切相关。我们的结论是，配子诱导蛋白质和蛋白质的合成是必不可少的细胞工会。在配子处理和未处理的细胞中合成的蛋白质也被分离和比较。考虑这些结果导致一个假设，大多数的配子诱导的蛋白质是膜蛋白正常合成，虽然在较少的量，在非共轭细胞和细胞获得的能力，团结当这些蛋白质积累在细胞表面上的一个有限的区域由另一个配子控制的机制。
The role of protein synthesis in the cell union was investigated in conjugation ofBlepharisma intermedium. In order to avoid possible complications due to the occurrence of other processes in conjugation, the homotypic cell union, in which conjugation is arrested at the stage of cell union without further changes, was used. Such unions were induced by treating cells of one mating type with the gamone of the other mating type for about 2 h. The induction of cell union was regularly accompanied by increased protein synthesis, which started 5 min after the beginning of the gamone treatment and continued for about 2 h. When protein synthesis was inhibited by cycloheximide, cell union was also inhibited. The extent of the two inhibitions were closely correlated. We concluded that gamone induces proteins and that protein synthesis is essential for cell union. Proteins synthesized in gamone-treated and non-treated cells were also separated and compared. Consideration of these results leads to a hypothesis that most of the gamone-induced proteins are membrane proteins normally synthesized, though in lesser amount, in non-conjugating cells and that cells gain the capacity to unite when these proteins are accumulated at a restricted area on the cell surface by another gamone-controlled mechanism.