Remodeling of adhesion and modulation of mechanical tensile forces during apoptosis in Drosophila epithelium
Remodeling of adhesion and modulation of mechanical tensile forces during apoptosis in Drosophila epithelium
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DOI：
10.1242/dev.139865
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发表时间：
2017-01
影响因子：
2.6
通讯作者：
Xiang Teng;L. Qin;R. Le Borgne;Y. Toyama
中科院分区：
文献类型：
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作者：
Xiang Teng;L. Qin;R. Le Borgne;Y. Toyama
细胞凋亡是一种在发育和组织稳态过程中清除受损或不必要细胞的机制。在组织内的细胞凋亡过程中，死亡细胞和邻近非死亡细胞之间的黏附需要重建，从而使凋亡细胞被排出体外。与此同时，在凋亡细胞和邻近细胞中形成的肌动球蛋白索的收缩驱动细胞挤压。迄今为止，细胞粘附动力学与凋亡细胞周围组织张力的进行性变化之间的协调尚未完全了解。组织细胞扩增是果蝇变形过程中一个协调的组织替换过程，其实时成像显示凋亡细胞和非死亡细胞之间的粘附连接（AJs）重塑，AJ成分（包括E-cadherin）水平降低。同时，周围组织张力被暂时释放。在相邻细胞中形成的超细胞肌动球蛋白索的收缩，将相邻细胞聚集在一起，并进一步重塑组织张力，以完成挤压。我们提出了一个模型，其中组织张力的调节代表了凋亡细胞挤压的机制。摘要：在果蝇上皮中，粘附连接成分水平降低时张力的短暂释放有助于细胞挤压凋亡。
Apoptosis is a mechanism of eliminating damaged or unnecessary cells during development and tissue homeostasis. During apoptosis within a tissue, the adhesions between dying and neighboring non-dying cells need to be remodeled so that the apoptotic cell is expelled. In parallel, contraction of actomyosin cables formed in apoptotic and neighboring cells drives cell extrusion. To date, the coordination between the dynamics of cell adhesion and the progressive changes in tissue tension around an apoptotic cell is not fully understood. Live imaging of histoblast expansion, which is a coordinated tissue replacement process during Drosophila metamorphosis, shows remodeling of adherens junctions (AJs) between apoptotic and non-dying cells, with a reduction in the levels of AJ components, including E-cadherin. Concurrently, surrounding tissue tension is transiently released. Contraction of a supra-cellular actomyosin cable, which forms in neighboring cells, brings neighboring cells together and further reshapes tissue tension toward the completion of extrusion. We propose a model in which modulation of tissue tension represents a mechanism of apoptotic cell extrusion. Summary: The transient release of tension upon a reduction in the levels of adherens junctions components contributes to apoptotic cell extrusion in Drosophila epithelium.