Renal angiotensin II concentration and interstitial infiltration of immune cells are correlated with blood pressure levels in salt-sensitive hypertension
Renal angiotensin II concentration and interstitial infiltration of immune cells are correlated with blood pressure levels in salt-sensitive hypertension
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DOI：
10.1152/ajpregu.00645.2006
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发表时间：
2007-07-01
影响因子：
2.8
通讯作者：
Rodriguez-Iturbe, Bernardo
中科院分区：
文献类型：
--
作者：
Franco, Martha;Martinez, Flavio;Rodriguez-Iturbe, Bernardo
肾免疫细胞浸润和肾小管间质区表达血管紧张素II （AII）的细胞是盐敏感性高血压（SSHTN）实验模型的特征。高盐摄入往往会抑制循环All水平，但在SSHTN中尚未研究All的肾内浓度。本研究探讨了这些特征之间的关系，以深入了解SSHTN的病理生理。用短时间内给予霉酚酸酯（MMF）的大鼠（n = 10）和未给予的大鼠（n = 11），以及饲喂高盐饮食（n = 7）和正常饮食（n = 11）的对照大鼠，在高盐饮食（HSD） 5周后，测定血浆血管紧张素II （AII）和肾间质AII（微透析技术）以及巨噬细胞、淋巴细胞和AII阳性细胞的浸润情况。与以往研究一样，MMF不影响aii相关性高血压，但可降低aii后的间质炎症和SSHTN。在HSD期间，未经MMF治疗的All组平均+/- SD低（2.4 +/- 1.4 pg/ml）和高间质AII浓度（1310 +/- 208 pg/ml）；MMF治疗导致间质AII显著降低（454 +/- 128 pg/ml）。肾All浓度与肾小管间质aii阳性细胞数量呈正相关。血压与间质AII呈正相关，与血浆AII呈负相关，因此提供了令人信服的证据，证明肾内All在AII诱导的盐驱动高血压模型中起着重要作用。
Renal immune cell infiltration and cells expressing angiotensin II (AII) in tubulointerstitial areas of the kidney are features of experimental models of salt-sensitive hypertension (SSHTN). A high-salt intake tends to suppress circulating All levels, but intrarenal concentrations of All have not been investigated in SSHTN. This study explored the relationship between these features to gain insight into the pathophysiology of SSHTN. Plasma angiotensin II (AII) and renal interstitial AII (microdialysis technique) and the infiltration of macrophages, lymphocytes, and AII-positive cells were determined in SSHTN induced by 5 wk of a high-salt diet (HSD) after short-term infusion of AII in rats with (n = 10) and without (n = 11) treatment with mycophenolate mofetil (MMF) and in control rats fed a high- (n = 7) and normal (n = 11) salt diet. As in previous studies, MMF did not affect AII-associated hypertension but reduced the interstitial inflammation and the SSHTN in the post-AII-period. During the HSD period, the All group untreated with MMF had mean +/- SD) low plasma (2.4 +/- 1.4 pg/ml) and high interstitial AII concentration (1,310 +/- 208 pg/ml); MMF treatment resulted in a significantly lower interstitial AII (454 +/- 128 pg/ml). Renal All concentration and the number of tubulointerstitial AII-positive cells were correlated. Blood pressure correlated positively with interstitial AII and negatively with plasma AII, thus giving compelling evidence of the paramount role of the All within the kidney in the AII-induced model of salt-driven hypertension.