The inflammation and estrogen metabolism impacts of polychlorinated biphenyls on endometrial cancer cells
The inflammation and estrogen metabolism impacts of polychlorinated biphenyls on endometrial cancer cells
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多氯联苯对子宫内膜癌细胞炎症和雌激素代谢的影响
DOI：
10.1016/j.tiv.2014.11.008
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发表时间：
2015-03-01
影响因子：
3.2
通讯作者：
Dong, Sijun
中科院分区：
文献类型：
--
作者：
Chen, Yajie;Huang, Qiansheng;Dong, Sijun
多氯联苯 (PCB) 是一种持久性和生物累积性化学品，会引起多种毒性作用。 PCBs对生殖系统的不利影响备受关注，但PCBs对子宫内膜的影响仍不清楚。在该研究中，子宫内膜腺癌Ishikawa细胞分别暴露于标称浓度0.3、3和30μM的二恶英类CB126和非二恶英类CB153，检测PCBs处理后的炎症和内分泌效应。结果显示，CB126在0.3和3μM的较低浓度下刺激Ishikawa细胞的增殖。相比之下，CBI 53不影响细胞的活力。两种同系物均对 SOD1 的酶活性产生刺激作用。 CB126 在 mRNA 和蛋白质水平上降低了 Interleukin-8 的丰度。拮抗剂对雌激素受体或芳烃受体的阻断消除了CB126对炎症因子表达的影响。暴露于较低剂量的 PCB 后，睾酮和 17β-雌二醇的水平没有变化。相应地，PCBs 不会影响雌激素代谢相关基因的 mRNA 表达。总之，我们的研究表明，PCB 通过 ER 和 AHR 受体影响炎症因子的表达，但没有观察到对雌激素代谢的毒性作用。 (C) 2014 Elsevier Ltd. 保留所有权利。
Polychlorinated biphenyls (PCBs) are persistent and bio-accumulative chemicals that provoke a wide range of toxic effects. Their adverse impacts on the reproductive system are of great concern, however, the effects of PCBs on endometrium are still unclear. In the study, the endometrial adenocarcinoma Ishikawa cells were exposed to both dioxin-like CB126 and non-dioxin-like CB153 at the nominal concentrations of 0.3, 3, and 30 mu M. The inflammatory and endocrine effects were detected after treatment by PCBs. Results showed that CB126 stimulated the proliferation of Ishikawa cells at lower concentrations of 0.3 and 3 mu M. By contrast, CBI 53 did not affect the viability of the cells. Both congeners exerted the stimulatory effects on the enzymatic activity of SOD1. CB126 decreased the abundance of Interleukin-8 both at the mRNA and protein levels. Blocking of estrogen receptor or aryl hydrocarbon receptor by the antagonist abolished the effects of CB126 on the expressions of inflammatory factors. The levels of testosterone and 17beta-estradiol were not changed after exposure to lower doses of PCBs. In accordance, PCBs did not affect the mRNA expressions of estrogen metabolism-related genes. In all, our study revealed that PCBs affected the expression of inflammatory factors through ER and AHR receptors, however, no toxic effects were observed on estrogen metabolism. (C) 2014 Elsevier Ltd. All rights reserved.